Počet záznamů: 1

Total synthesis of [N-15]-labelled C6-substituted purines from [N-15]-formamide-easy preparation of isotopically labelled cytokinins and derivatives

SYSNO ASEP	0498271
Druh ASEP	J - Článek v odborném periodiku
Zařazení RIV	J - Článek v odborném periodiku
Poddruh J	Článek ve WOS
Název	Total synthesis of [N-15]-labelled C6-substituted purines from [N-15]-formamide-easy preparation of isotopically labelled cytokinins and derivatives
Tvůrce(i)	Buček, J. (CZ) Zatloukal, M. (CZ) Havlíček, Libor (UEB-Q)_{RID, ORCID} Plíhalová, Lucie (UEB-Q)_{ORCID, RID} Pospíšil, T. (CZ) Novák, Ondřej (UEB-Q)_{RID, ORCID, SAI} Doležal, Karel (UEB-Q)_{RID, ORCID} Strnad, Miroslav (UEB-Q)_{RID, ORCID}
Celkový počet autorů	8
Číslo článku	181322
Zdroj.dok.	Royal Society Open Science. - : Royal Society Publishing - ISSN 2054-5703 Roč. 5, č. 11 (2018)
Poč.str.	14 s.
Jazyk dok.	eng - angličtina
Země vyd.	GB - Velká Británie
Klíč. slova	cell-division ; biological-activity ; aromatic cytokinins ; plant-tissues ; adenine ; zeatin ; identification ; diphosphate ; mechanism ; thaliana ; synthesis ; N-15-labelled ; purine ; cytokinin
Vědní obor RIV	ED - Fyziologie
Obor OECD	Biochemical research methods
CEP	GA16-04184S GA ČR - Grantová agentura ČR
	LO1204 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy
	EF16_019/0000738 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy
Institucionální podpora	UEB-Q - RVO:61389030
UT WOS	000451777500038
DOI	10.1098/rsos.181322
Anotace	Cytokinins (CKs) and their metabolites and derivatives are essential for cell division, plant growth regulation and development. They are typically found at minute concentrations in plant tissues containing very complicated biological matrices. Therefore, defined standards labelled with stable isotopes are required for precise metabolic profiling and quantification of CKs, as well as in vivo elucidation of CK biosynthesis in various plant species. In this work, 11 [N-15]-labelled C6-purine derivatives were prepared, among them 5 aromatic (4, 5, 6, 7, 8) and 3 isoprenoid (9, 10, 11) CKs. Compared to current methods, optimized syntheses of 6-amino-9H-[N-15(5)]-purine (adenine) and 6-chloro-9H-[N-15(4)]-purine (6-chloropurine) were performed to achieve more effective, selective and generally easier approaches. The chemical identity and purity of prepared compounds were confirmed by physico-chemical analyses (TLC, HRMS, HPLC-MS, H-1, C-13, N-15 NMR). The presented approach is applicable for the synthesis of any other desired [N-15(4) ]-labelled C6-substituted purine derivatives.
Pracoviště	Ústav experimentální botaniky
Kontakt	David Klier, knihovna@ueb.cas.cz, Tel.: 220 390 469
Rok sběru	2019

Počet záznamů: 1