Počet záznamů: 1
Variable regions of the glyS, infB and rplB genes usable as novel genetic markers for identification and phylogenetic purposes of genera belonging to the family Propionibacteriaceae
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SYSNO ASEP 0495885 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Variable regions of the glyS, infB and rplB genes usable as novel genetic markers for identification and phylogenetic purposes of genera belonging to the family Propionibacteriaceae Tvůrce(i) Mekadim, Chahrazed (UZFG-Y) ORCID
Killer, Jiří (UZFG-Y) RID, ORCID
Pechar, R. (CZ)
Mrázek, Jakub (UZFG-Y) RID, ORCIDZdroj.dok. International Journal of Systematic and Evolutionary Microbiology. - : Microbiology Society - ISSN 1466-5026
Roč. 68, č. 8 (2018), s. 2697-2705Poč.str. 9 s. Forma vydání Tištěná - P Jazyk dok. eng - angličtina Země vyd. GB - Velká Británie Klíč. slova Propionibacteriaceae ; glyS, infB and rplB genes ; phylogenetics Vědní obor RIV EE - Mikrobiologie, virologie Obor OECD Microbiology CEP EF15_003/0000460 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy NV16-27449A GA MZd - Ministerstvo zdravotnictví Způsob publikování Open access Institucionální podpora UZFG-Y - RVO:67985904 UT WOS 000440971600044 EID SCOPUS 85051184012 DOI 10.1099/ijsem.0.002873 Anotace No common, unique genetic markers applicable to classification and phylogenetics for significant genera within the Propionibacteriaceae family have been suggested yet. Therefore, the aim of the study was to propose those genes in the genera Acidipropionibacterium, Cutibacterium, Propionibacterium and Pseudopropionibacterium. These genera were recently elicited from the genus Propionibacterium through whole genomic analyses. Three housekeeping genes, glyS, infB and rplB, were selected from many others according to the requirements for appropriate classification/phylogenetic markers. Concrete fragments of the genes were amplified using specific primers in most of the type (14) and 11 wild strains (originating from dairy products, human skin and the crop of a laying hen) recently classified into the genus Propionibacterium. Sequences obtained from amplicons were used to perform gene statistics and phylogenetic analyses with respect to applicability in classification, typing and phylogeny. The 16S rRNA gene sequences, still considered relevant in spite of its proven shortcomings as a basic tool for evaluation of bacterial phylogeny, were used as a baseline for comparative analyses. The statistics of the gene sequences revealed that the variable regions of all three genes have higher resolution capabilities among strains examined compared to the 16S rRNA gene analysis. Phylogenetic analyses based on individual gene sequences and their concatenate enabled to distinguish clusters of species belonging to the genera Acidipropionibacterium, Cutibacterium and Propionibacterium, which corresponds with a recently reported genomic study. Thus, the crucial importance of this study is the economically advantageous classification and typing of propionibacterial isolates and strains through the three gene regions in contrast to the requirement for whole genomic assays. Pracoviště Ústav živočišné fyziologie a genetiky Kontakt Jana Zásmětová, knihovna@iapg.cas.cz, Tel.: 315 639 554 Rok sběru 2019 Elektronická adresa https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijsem.0.002873
Počet záznamů: 1