Počet záznamů: 1
Characterization and annotation of oxidized glycerophosphocholines for non-targeted metabolomics with LC-QTOF-MS data
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SYSNO ASEP 0495424 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Characterization and annotation of oxidized glycerophosphocholines for non-targeted metabolomics with LC-QTOF-MS data Tvůrce(i) de la Fuente, A.G. (ES)
Traldi, F. (ES)
Široká, Jitka (UEB-Q) ORCID
Kretowski, A. (PL)
Ciborowski, M. (PL)
Otero, A. (CZ)
Barbas, C. (ES)
Godzien, J. (ES)Celkový počet autorů 8 Zdroj.dok. Analytica Chimica Acta. - : Elsevier - ISSN 0003-2670
Roč. 1037, DEC 11 (2018), s. 358-368Poč.str. 11 s. Jazyk dok. eng - angličtina Země vyd. NL - Nizozemsko Klíč. slova mass-spectrometry ; phospholipid oxidation ; identification ; inflammation ; products ; cells ; hdl ; Non-targeted metabolomics ; Identification ; Annotation ; Oxidized glycerophosphocholines ; oxPAPC ; lc-esi-qtof Vědní obor RIV EB - Genetika a molekulární biologie Obor OECD Cell biology CEP LO1204 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy Institucionální podpora UEB-Q - RVO:61389030 UT WOS 000446225000036 EID SCOPUS 85052337967 DOI 10.1016/j.aca.2018.08.005 Anotace The biological role of oxidized glycerophosphocholines (oxPCs) is a current topic of research importantly contributing to the understanding of health and disease. Global non-targeted metabolomics offers an interesting approach to expand current knowledge and link oxPCs to new biological functions. Although this strategy is successful, it also has some limitations which are clearly noticeable during the identification process. For this reason, clear rules related to the identification of each group of metabolites are needed. This work attempts to provide the reader with a guideline for the recognition and annotation of oxidation among phosphocholines (PCs). Using several chromatographic characteristics and spectral information from tandem mass spectrometry, rapid and reliable annotation of long and short chain oxPCs can be performed. Some of this knowledge has been implemented in the publicly available annotation tool 'CEU Mass Mediator' (CMM) for semi-automated assignment of oxidation. Additionally, this tool was supplemented with accurate monoisotopic masses of oxPCs, expanding current information in other databases. Moreover, the characterization of oxidization products of PC(16:0/20:4) known as PAPC has been performed, providing a list of accurate mass product ions and neutral losses. Pracoviště Ústav experimentální botaniky Kontakt David Klier, knihovna@ueb.cas.cz, Tel.: 220 390 469 Rok sběru 2019
Počet záznamů: 1