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Macrophage sensing of single-walled carbon nanotubes via Toll-like receptors
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SYSNO ASEP 0494959 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Macrophage sensing of single-walled carbon nanotubes via Toll-like receptors Tvůrce(i) Mukherjee, S.P. (SE)
Bondarenko, O. (SE)
Kohonen, P. (SE)
Andon, F.T. (SE)
Brzicová, Táňa (UEM-P)
Gessner, A. (DE)
Mathur, S. (DE)
Bottini, M. (IT)
Calligari, P. (IT)
Stella, L. (IT)
Kisin, E. (US)
Shvedová, A. (US)
Autio, R. (FI)
Salminen-Mankonen, H. (FI)
Lahesmaa, R. (FI)
Fadeel, B. (SE)Číslo článku 1115 Zdroj.dok. Scientific Reports. - : Nature Publishing Group - ISSN 2045-2322
Roč. 8, jan (2018)Poč.str. 17 s. Jazyk dok. eng - angličtina Země vyd. GB - Velká Británie Klíč. slova pattern-recognition receptors ; graphene oxide ; protein corona ; immune-system Vědní obor RIV EB - Genetika a molekulární biologie Obor OECD Cell biology Institucionální podpora UEM-P - RVO:68378041 UT WOS 000422739300078 EID SCOPUS 85040925860 DOI 10.1038/s41598-018-19521-9 Anotace Carbon-based nanomaterials including carbon nanotubes (CNTs) have been shown to trigger inflammation. However, how these materials are 'sensed' by immune cells is not known. Here we compared the effects of two carbon-based nanomaterials, single-walled CNTs (SWCNTs) and graphene oxide (GO), on primary human monocyte-derived macrophages. Genome-wide transcriptomics assessment was performed at sub-cytotoxic doses. Pathway analysis of the microarray data revealed pronounced effects on chemokine-encoding genes in macrophages exposed to SWCNTs, but not in response to GO, and these results were validated by multiplex array-based cytokine and chemokine profiling. Conditioned medium from SWCNT-exposed cells acted as a chemoattractant for dendritic cells. Chemokine secretion was reduced upon inhibition of NF-kappa B, as predicted by upstream regulator analysis of the transcriptomics data, and Toll-like receptors (TLRs) and their adaptor molecule, MyD88 were shown to be important for CCL5 secretion. Moreover, a specific role for TLR2/4 was confirmed by using reporter cell lines. Computational studies to elucidate how SWCNTs may interact with TLR4 in the absence of a protein corona suggested that binding is guided mainly by hydrophobic interactions. Taken together, these results imply that CNTs may be 'sensed' as pathogens by immune cells. Pracoviště Ústav experimentální medicíny Kontakt Lenka Koželská, lenka.kozelska@iem.cas.cz, Tel.: 241 062 218, 296 442 218 Rok sběru 2019
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