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Spectrophotometric Determination of Phycobiliprotein Content in Cyanobacterium Synechocystis
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SYSNO ASEP 0493286 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Spectrophotometric Determination of Phycobiliprotein Content in Cyanobacterium Synechocystis Tvůrce(i) Zavřel, Tomáš (UEK-B) RID, SAI, ORCID
Chmelík, Dominik (UEK-B) SAI
Sinětova, Maria A. (UEK-B)
Červený, Jan (UEK-B) RID, ORCID, SAIČíslo článku e58076 Zdroj.dok. Jove-Journal of Visualized Experiments - ISSN 1940-087X
Roč. 139, sep (2018)Poč.str. 9 s. Jazyk dok. eng - angličtina Země vyd. US - Spojené státy americké Klíč. slova Phycobilisomes ; phycobilins ; phycocyanobilins ; phycocyanin ; allophycocyanin ; pigments ; light harvesting ; protein ; cyanobacteria ; algae ; spectrophotometry ; extraction Vědní obor RIV CE - Biochemie Obor OECD Biochemistry and molecular biology CEP LO1415 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy GA18-24397S GA ČR - Grantová agentura ČR LM2015055 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy Institucionální podpora UEK-B - RVO:86652079 UT WOS 000456210900004 EID SCOPUS 85054377142 DOI 10.3791/58076 Anotace This is a simple protocol for the quantitative determination of phycobiliprotein content in the model cyanobacterium Synechocystis. Phycobiliproteins are the most important components of phycobilisomes, the major light-harvesting antennae in cyanobacteria and several algae taxa. The phycobilisomes of Synechocystis contain two phycobiliproteins: phycocyanin and allophycocyanin. This protocol describes a simple, efficient, and reliable method for the quantitative determination of both phycocyanin and allophycocyanin in this model cyanobacterium. We compared several methods of phycobiliprotein extraction and spectrophotometric quantification. The extraction procedure as described in this protocol was also successfully applied to other cyanobacteria strains such as Cyanothece sp., Synechococcuselongatus, Spirulina sp., Arthrospira sp., and Nostoc sp., as well as to red algae Porphyridium cruentum. However, the extinction coefficients of specific phycobiliproteins from various taxa can differ and it is, therefore, recommended to validate the spectrophotometric quantification method for every single strain individually. The protocol requires little time and can be performed in any standard life science laboratory since it requires only standard equipment. Pracoviště Ústav výzkumu globální změny Kontakt Nikola Šviková, svikova.n@czechglobe.cz, Tel.: 511 192 268 Rok sběru 2019
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