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Capillary Electromigration Separation Methods
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SYSNO ASEP 0492951 Druh ASEP M - Kapitola v monografii Zařazení RIV C - Kapitola v knize Název Application of capillary electromigration methods for physicochemical measurements Tvůrce(i) Štěpánová, Sille (UOCHB-X) ORCID
Kašička, Václav (UOCHB-X) RID, ORCIDZdroj.dok. Capillary Electromigration Separation Methods. - Amsterdam : Elsevier, 2018 / Poole Colin F. - ISBN 978-0-12-809375-7 Rozsah stran s. 547-591 Poč.str. 45 s. Poč.str.knihy 626 Forma vydání Tištěná - P Jazyk dok. eng - angličtina Země vyd. NL - Nizozemsko Klíč. slova acidity constant ; binding constant ; capillary electrophoresis ; effective charge ; electrophoretic mobility Vědní obor RIV CB - Analytická chemie, separace Obor OECD Analytical chemistry CEP GA15-01948S GA ČR - Grantová agentura ČR GA17-10832S GA ČR - Grantová agentura ČR Institucionální podpora UOCHB-X - RVO:61388963 UT WOS 000462135300022 DOI 10.1016/B978-0-12-809375-7.00024-1 Anotace In this chapter, high-performance capillary electromigration (HPCE or CE) methods are presented as powerful tools for the determination of important physicochemical parameters of (bio)molecules. Using the different HPCE methods (zone electrophoresis in free solutions or in sieving/gel media, isotachophoresis, isoelectric focusing, affinity electrophoresis, and electrokinetic chromatography), the following physicochemical characteristics of a wide spectrum of compounds can be determined: effective, ionic and limiting mobilities, effective charges, isoelectric points, Stokes radii, relative molecular masses, partition constants, diffusion coefficients, and acidity (ionization) constants (pKa). In addition, the binding (stability, association, formation, or dissociation) constants of (bio)molecular complexes and rate constants of chemical and enzymatic reactions and noncovalent molecular interactions can be estimated. Advantages of HPCE methods for these physicochemical measurements include high separation efficiency, short analysis time, and especially the ultra-small mass (picogram range) and volume (nanoliter level) of injected solute samples and low consumption of chemicals and solvents. Moreover, the characterization of not quite pure solutes and solutes sparingly soluble in water is possible. Pracoviště Ústav organické chemie a biochemie Kontakt asep@uochb.cas.cz ; Kateřina Šperková, Tel.: 232 002 584 ; Viktorie Chládková, Tel.: 232 002 434 Rok sběru 2019
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