Počet záznamů: 1
The Effect of Human Mesenchymal Stem Cells Derived from Wharton's Jelly in Spinal Cord Injury Treatment Is Dose-Dependent and Can Be Facilitated by Repeated Application
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SYSNO ASEP 0492882 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název The Effect of Human Mesenchymal Stem Cells Derived from Wharton's Jelly in Spinal Cord Injury Treatment Is Dose-Dependent and Can Be Facilitated by Repeated Application Tvůrce(i) Krůpa, Petr (UEM-P) RID, ORCID
Vacková, Irena (UEM-P)
Růžička, Jiří (UEM-P)
Závišková, Kristýna (UEM-P)
Dubišová, Jana (UEM-P)
Kočí, Zuzana (UEM-P)
Turnovcová, Karolína (UEM-P) ORCID
Machová-Urdzíková, Lucia (UEM-P) ORCID
Kubinová, Šárka (UEM-P) RID, ORCID
Řehák, S. (CZ)
Jendelová, Pavla (UEM-P) RID, ORCIDČíslo článku 1503 Zdroj.dok. International Journal of Molecular Sciences. - : MDPI
Roč. 19, č. 5 (2018)Poč.str. 18 s. Jazyk dok. eng - angličtina Země vyd. CH - Švýcarsko Klíč. slova spinal cord injury ; human mesenchymal stem cells ; Wharton's jelly ; inflammatory response Vědní obor RIV EB - Genetika a molekulární biologie Obor OECD Neurosciences (including psychophysiology CEP EF15_003/0000419 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy GBP304/12/G069 GA ČR - Grantová agentura ČR GA17-03765S GA ČR - Grantová agentura ČR LM2015064 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy ED1.1.00/02.0109 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy LQ1604 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy Institucionální podpora UEM-P - RVO:68378041 UT WOS 000435297000239 EID SCOPUS 85047250976 DOI 10.3390/ijms19051503 Anotace Human mesenchymal stem cells derived from Wharton's jelly (WJ-MSCs) were used for the treatment of the ischemic-compression model of spinal cord injury in rats. To assess the effectivity of the treatment, different dosages (0.5 or 1.5 million cells) and repeated applications were compared. Cells or saline were applied intrathecally by lumbar puncture for one week only, or in three consecutive weeks after injury. Rats were assessed for locomotor skills (BBB, rotarod, flat beam) for 9 weeks. Spinal cord tissue was morphometrically analyzed for axonal sprouting, sparing of gray and white matter and astrogliosis. Endogenous gene expression (Gfap, Casp3, Irf5, Cd86, Mrc1, Cd163) was studied with quantitative Real-time polymerase chain reaction (qRT PCR). Significant recovery of functional outcome was observed in all of the treated groups except for the single application of the lowest number of cells. Histochemical analyses revealed a gradually increasing effect of grafted cells, resulting in a significant increase in the number of GAP43+ fibers, a higher amount of spared gray matter and reduced astrogliosis. mRNA expression of macrophage markers and apoptosis was downregulated after the repeated application of 1.5 million cells. We conclude that the effect of hWJ-MSCs on spinal cord regeneration is dose-dependent and potentiated by repeated application. Pracoviště Ústav experimentální medicíny Kontakt Lenka Koželská, lenka.kozelska@iem.cas.cz, Tel.: 241 062 218, 296 442 218 Rok sběru 2019
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