Počet záznamů: 1
p73, like its p53 homolog, shows preference for inverted repeats forming cruciforms
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SYSNO ASEP 0492335 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název p73, like its p53 homolog, shows preference for inverted repeats forming cruciforms Tvůrce(i) Čechová, Jana (BFU-R)
Coufal, Jan (BFU-R) ORCID
Jagelská, Eva (BFU-R)
Fojta, Miroslav (BFU-R) RID, ORCID
Brázda, Václav (BFU-R) RID, ORCIDCelkový počet autorů 5 Číslo článku e0195835 Zdroj.dok. PLoS ONE. - : Public Library of Science - ISSN 1932-6203
Roč. 13, č. 4 (2018)Poč.str. 13 s. Jazyk dok. eng - angličtina Země vyd. US - Spojené státy americké Klíč. slova sequence-specific binding ; dna-binding ; transcriptional activity ; supercoiled dna Vědní obor RIV CE - Biochemie Obor OECD Biochemistry and molecular biology CEP GA15-21855S GA ČR - Grantová agentura ČR EF15_003/0000477 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy Institucionální podpora BFU-R - RVO:68081707 UT WOS 000430290200060 DOI 10.1371/journal.pone.0195835 Anotace p73 is a member of the p53 protein family and has essential functions in several signaling pathways involved in development, differentiation, DNA damage responses and cancer. As a transcription factor, p73 achieves these functions by binding to consensus DNA sequences and p73 shares at least partial target DNA binding sequence specificity with p53. Transcriptional activation by p73 has been demonstrated for more than fifty p53 targets in yeast and/or human cancer cell lines. It has also been shown previously that p53 binding to DNA is strongly dependent on DNA topology and the presence of inverted repeats that can form DNA cruciforms, but whether p73 transcriptional activity has similar dependence has not been investigated. Therefore, we evaluated p73 binding to a set of p53-response elements with identical theoretical binding affinity in their linear state, but different probabilities to form extra helical structures. We show by a yeast-based assay that transactivation in vivo correlated more with the relative propensity of a response element to form cruciforms than to its expected in vitro DNA binding affinity. Structural features of p73 target sites are therefore likely to be an important determinant of its transactivation function. Pracoviště Biofyzikální ústav Kontakt Jana Poláková, polakova@ibp.cz, Tel.: 541 517 244 Rok sběru 2019
Počet záznamů: 1