Počet záznamů: 1
Capillary Electromigration Separation Methods
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SYSNO ASEP 0491723 Druh ASEP M - Kapitola v monografii Zařazení RIV C - Kapitola v knize Název Capillary Gel and Sieving Electrophoresis Tvůrce(i) Mikšík, Ivan (FGU-C) RID, ORCID, SAI Zdroj.dok. Capillary Electromigration Separation Methods. - Amsterdam : Elsevier, 2018 / Poole Colin F. - ISBN 978-0-12-809375-7 Rozsah stran s. 143-165 Poč.str. 23 s. Poč.str.knihy 626 Forma vydání Tištěná - P Jazyk dok. eng - angličtina Země vyd. NL - Nizozemsko Klíč. slova capillary gel electrophoresis ; nucleic acid analysis ; DNA analysis ; protein analysis ; lab-on-a-chip ; miniaturization Vědní obor RIV CB - Analytická chemie, separace Obor OECD Analytical chemistry CEP GA17-10832S GA ČR - Grantová agentura ČR Institucionální podpora FGU-C - RVO:67985823 UT WOS 000462135300006 DOI 10.1016/B978-0-12-809375-7.00006-X Anotace Capillary gel electrophoresis (CGE) is a powerful method for analysis of biopolymers such as DNA, RNA, and proteins by sieving phenomena, according to size (molecular weight). This technique evolved from “traditional” gel electrophoresis but has many advantages, such as automation, high separation efficiency, on-capillary detection, anticonvective nature of the capillary, and array dimension (multicapillary system). An interesting improvement is also miniaturization, lab-on-a-chip should also be emphasized, including two-dimensional separation. All these properties and technical properties are reviewed. Special attention is paid to gels used (polymers), their preparation, and applicability. Particular parts of the chapter are examples of applications of CGE for analysis of real samples. Commercially available resources, such as instruments and kits, are also reviewed. Pracoviště Fyziologický ústav Kontakt Lucie Trajhanová, lucie.trajhanova@fgu.cas.cz, Tel.: 241 062 400 Rok sběru 2019
Počet záznamů: 1