Počet záznamů: 1
Modification of a Mercury Electrode with Different Thioalkanes: Structure-Sensitive Bovine Serum Albumin Analysis
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SYSNO ASEP 0491232 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Modification of a Mercury Electrode with Different Thioalkanes: Structure-Sensitive Bovine Serum Albumin Analysis Tvůrce(i) Ostatná, Veronika (BFU-R) RID, ORCID
Černocká, Hana (BFU-R) RID, ORCID
Hasoň, Stanislav (BFU-R) RID, ORCID
Paleček, Emil (BFU-R) RID, ORCIDCelkový počet autorů 4 Zdroj.dok. ChemElectroChem. - : Wiley - ISSN 2196-0216
Roč. 5, č. 10 (2018), s. 1373-1379Poč.str. 7 s. Forma vydání Tištěná - P Jazyk dok. eng - angličtina Země vyd. DE - Německo Klíč. slova self-assembled monolayers ; electroanalytical chemistry ; electrochemical detection ; amalgam electrodes Vědní obor RIV CG - Elektrochemie Obor OECD Electrochemistry (dry cells, batteries, fuel cells, corrosion metals, electrolysis) CEP GA18-18154S GA ČR - Grantová agentura ČR Institucionální podpora BFU-R - RVO:68081707 UT WOS 000431974500006 DOI 10.1002/celc.201800275 Anotace Chronopotentiometric stripping (CPS) in combination with mercury electrodes is convenient for structure-sensitive protein analysis. By using this method, we tested the discrimination between native and denatured bovine serum albumin (BSA) at various alkanethiol self-assembled monolayers (SAMs). Our results showed that alkanethiol SAMs with different headgroups or different chain lengths had different influences on the efficiency of CPS in protecting BSA from the electric field-induced denaturation. SAMs with longer alkanethiol chain lengths were more efficient in protecting BSA from denaturation at negatively charged electrode surface than those formed by short-chain alkanethiols. Short alkanethiol SAMs could be prepared by co-adsorption with the protein on the electrode surface, which was not possible with the long-chain alkanethiols. CPS data were supported by impedance analysis. We believe that our work will be useful in the electrochemical sensing of nucleic acid-protein and protein-protein binding. Pracoviště Biofyzikální ústav Kontakt Jana Poláková, polakova@ibp.cz, Tel.: 541 517 244 Rok sběru 2019
Počet záznamů: 1