Počet záznamů: 1
Capillary Network Morphometry of Pig Soleus Muscle Significantly Changes in 24 Hours After Death
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SYSNO ASEP 0490241 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Capillary Network Morphometry of Pig Soleus Muscle Significantly Changes in 24 Hours After Death Tvůrce(i) Eržen, I. (SI)
Janáček, Jiří (FGU-C) RID, ORCID
Kreft, M. (SI)
Kubínová, Lucie (FGU-C) RID, ORCID
Cvetko, E. (SI)Zdroj.dok. Journal of Histochemistry and Cytochemistry. - : Sage - ISSN 0022-1554
Roč. 66, č. 1 (2018), s. 23-31Poč.str. 9 s. Jazyk dok. eng - angličtina Země vyd. US - Spojené státy americké Klíč. slova capillaries ; confocal microscopy ; image analysis ; pig ; postmortem changes ; skeletal muscle Vědní obor RIV FP - Ostatní lékařské obory Obor OECD Anatomy and morphology (plant science to be 1.6) CEP LM2015062 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy Institucionální podpora FGU-C - RVO:67985823 UT WOS 000418835500002 EID SCOPUS 85039732994 DOI 10.1369/0022155417737061 Anotace Capillary network characteristics are invaluable for diagnostics of muscle diseases. Biopsy material is limited in size and mostly not accessible for intensive research. Therefore, especially in human tissue, studies are performed on autopsy material. To approach the problem whether it is reliable to deduce hypotheses from autopsy material to explain physiological and pathological processes, we studied capillarity in pig soleus muscle 1 and 24 hr after death. Capillaries and muscle fibers were immunofluorescently marked, and images were acquired with a confocal microscope. Characteristics of the capillary network were estimated by image analysis methods using several plugins of the Ellipse program. Twenty-four hours after death, the measured characteristics of the capillary network differ by up to 50% when compared with samples excised 1 hr after death. Muscle fiber diameter, the measured capillary length, and tortuosity were reduced, and capillary network became more anisotropic. The main postmortem change that affects capillaries is evidently geometric deformation of muscle tissue. In conclusion, when comparing results from biopsy samples with those from autopsy samples, the effect of postmortem changes on the measured parameters must be carefully considered. Pracoviště Fyziologický ústav Kontakt Lucie Trajhanová, lucie.trajhanova@fgu.cas.cz, Tel.: 241 062 400 Rok sběru 2019
Počet záznamů: 1