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Potential of the strain Raoultella sp KDF8 for removal of analgesics
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SYSNO ASEP 0489382 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Potential of the strain Raoultella sp KDF8 for removal of analgesics Tvůrce(i) Palyzová, Andrea (MBU-M) RID, ORCID
Zahradník, Jiří (BTO-N)
Marešová, Helena (MBU-M) RID, ORCID
Sokolová, Lucie (MBU-M)
Kyslíková, Eva (MBU-M)
Grulich, Michal (MBU-M) RID
Štěpánek, Václav (MBU-M) RID, ORCID
Řezanka, Tomáš (MBU-M) ORCID
Kyslík, Pavel (MBU-M) RID, ORCIDZdroj.dok. Folia Microbiologica. - : Springer - ISSN 0015-5632
Roč. 63, č. 3 (2018), s. 273-282Poč.str. 10 s. Jazyk dok. eng - angličtina Země vyd. NL - Nizozemsko Klíč. slova Microbial degradation ; Raoultella sp. ; Analgesics Vědní obor RIV EE - Mikrobiologie, virologie Obor OECD Microbiology Vědní obor RIV – spolupráce Biotechnologický ústav - Mikrobiologie, virologie CEP TH02030337 GA TA ČR - Technologická agentura ČR Institucionální podpora MBU-M - RVO:61388971 ; BTO-N - RVO:86652036 UT WOS 000430317100002 EID SCOPUS 85033434424 DOI 10.1007/s12223-017-0563-2 Anotace The bacterial strain KDF8 capable of growth in the presence of diclofenac and codeine analgesics was obtained after chemical mutagenesis of nature isolates from polluted soils. The strain KDF8 was identified as Raoultella sp. based on its morphology, biochemical properties, and 16S rRNA gene sequence. It was deposited in the Czech Collection of Microorganisms under the number CCM 8678. A growing culture efficiently removed diclofenac (92% removal) and partially also codeine (about 30% degradation) from culture supernatants within 72 h at 28 degrees C. The degradation of six analgesics by the whole cell catalyst was investigated in detail. The maximum degradation of diclofenac (91%) by the catalyst was achieved at pH(INI) of 7 (1 g/L diclofenac). The specific removal rate at high concentrations of diclofenac and codeine increased up to 16.5 mg/g(CDW) per h and 5.1 mg/g(CDW) per h, respectively. HPLC analysis identified 4'-hydroxydiclofenac as a major metabolite of diclofenac transformation and 14-hydroxycodeinone as codeine transformation product. The analgesics ibuprofen and ketoprofen were also removed, albeit to a lower extent of 3.2 and 2.0 mg/g(CDW) per h, respectively. Naproxen and mefenamic acid were not degraded. Pracoviště Mikrobiologický ústav Kontakt Eliška Spurná, eliska.spurna@biomed.cas.cz, Tel.: 241 062 231 Rok sběru 2019
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