Počet záznamů: 1

In vitro evaluation of the monoclonal antibody Cu-64-IgG M75 against human carbonic anhydrase IX and its in vivo imaging

SYSNO ASEP	0488728
Druh ASEP	J - Článek v odborném periodiku
Zařazení RIV	J - Článek v odborném periodiku
Poddruh J	Článek ve WOS
Název	In vitro evaluation of the monoclonal antibody Cu-64-IgG M75 against human carbonic anhydrase IX and its in vivo imaging
Tvůrce(i)	Čepa, Adam (UJF-V) Ráliš, Jan (UJF-V)_SAI Král, Vlastimil (UMG-J)_RID Paurová, M. (CZ) Kučka, Jan (UMCH-V)_{RID, ORCID} Humajová, J. (CZ) Lázníček, M. (CZ) Lebeda, Ondřej (UJF-V)_{RID, ORCID, SAI}
Celkový počet autorů	8
Zdroj.dok.	Applied Radiation and Isotopes. - : Elsevier - ISSN 0969-8043 Roč. 133, č. March (2018), s. 9-13
Poč.str.	5 s.
Forma vydání	Tištěná - P
Jazyk dok.	eng - angličtina
Země vyd.	GB - Velká Británie
Klíč. slova	IgG M75 ; immunoaffinity ; imaging ; monoclonal atibody ; Cu-64
Vědní obor RIV	FP - Ostatní lékařské obory
Obor OECD	Radiology, nuclear medicine and medical imaging
Vědní obor RIV – spolupráce	Ústav molekulární genetiky - Genetika a molekulární biologie Ústav makromolekulární chemie - Makromolekulární chemie
Institucionální podpora	UJF-V - RVO:61389005 ; UMG-J - RVO:68378050 ; UMCH-V - RVO:61389013
UT WOS	000425577300003
EID SCOPUS	85038854401
DOI	10.1016/j.apradiso.2017.12.013
Anotace	Specific oncology diagnostics requires new types of the selective radiophannaceuticals, particularly those suitable for the molecular PET imaging. The aim of this work is to present a new, specific PET-immunodiagnostic radiopharmaceutical based on the monoclonal antibody IgG M75 targeting human carbonic anhydrase IX labelled with Cu-64 (T-1/2 = 12.70 h) and its in vitro and in vivo evaluation. The antibody IgG M75 was conjugated with a non-commercial copper-specific chelator 'phosphinate' and then labelled with the positron emitter 64Cu. Stability of the labelled conjugated was tested in human serum. The immunoreactivity of the labelled conjugate was evaluated in vitro on a suitable cell cultures of the colorectal carcinoma (HT-29) and its imaging properties were estimated in vivo on a mouse model with inoculated colorectal carcinoma HT-29 imaged on a mu PET/CT. The tested radioimmunoconjugate was obtained in a specific activity of 0.25-0.5 MBq/mu g. In vitro uptake experiments revealed specific binding to the HT-29 cells (45 +/- 2.8% of the total added activity) and the measured K-D value was found to be 9.2 nM. Imaging clearly demonstrated significant uptake of the labelled monoclonal antibody in the tumour at 18 h post administration. The radioimmunoconjugate Cu-64-PS-IgG M75 seems to be a suitable candidate for PET diagnostics of hypoxic tumours expressing human carbonic anhydrase IX.
Pracoviště	Ústav jaderné fyziky
Kontakt	Markéta Sommerová, sommerova@ujf.cas.cz, Tel.: 266 173 228
Rok sběru	2019

Počet záznamů: 1