Počet záznamů: 1
Disruption of the C/EBP alpha-miR-182 balance impairs granulocytic differentiation
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SYSNO ASEP 0487338 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Disruption of the C/EBP alpha-miR-182 balance impairs granulocytic differentiation Tvůrce(i) Wurm, A.A. (DE)
Zjablovskaja, Polina (UMG-J)
Kardošová, Miroslava (UMG-J)
Gerloff, D. (DE)
Braeuer-Hartmann, D. (DE)
Katzerke, C. (DE)
Hartmann, J.U. (DE)
Benoukraf, T. (SG)
Fricke, S. (DE)
Hilger, N. (DE)
Mueller, A.M. (DE)
Bill, M. (DE)
Schwind, S. (DE)
Tenen, D.G. (US)
Niederwieser, D. (DE)
Alberich-Jorda, Meritxell (UMG-J) RID
Behre, G. (DE)Celkový počet autorů 17 Číslo článku 46 Zdroj.dok. Nature Communications. - : Nature Publishing Group
Roč. 8, červen (2017)Poč.str. 16 s. Jazyk dok. eng - angličtina Země vyd. GB - Velká Británie Klíč. slova acute myeloid-leukemia ; colony-stimulating factor ; acute promyelocytic leukemia ; hematopoietic stem-cells ; c/ebp-alpha function ; gene-expression ; cebpa mutations ; cycle control ; up-regulation ; in-vivo Vědní obor RIV EB - Genetika a molekulární biologie Obor OECD Cell biology Institucionální podpora UMG-J - RVO:68378050 UT WOS 000404333000005 DOI 10.1038/s41467-017-00032-6 Anotace Transcription factor C/EBP alpha is a master regulator of myelopoiesis and its inactivation is associated with acute myeloid leukemia. Deregulation of C/EBP alpha by microRNAs during granulopoiesis or acute myeloid leukemia development has not been studied. Here we show that oncogenic miR-182 is a strong regulator of C/EBP alpha. Moreover, we identify a regulatory loop between C/EBP alpha and miR-182. While C/EBP alpha blocks miR-182 expression by direct promoter binding during myeloid differentiation, enforced expression of miR-182 reduces C/EBP alpha protein level and impairs granulopoiesis in vitro and in vivo. In addition, miR-182 expression is highly elevated particularly in acute myeloid leukemia patients with C-terminal CEBPA mutations, thereby depicting a mechanism by which C/EBP alpha blocks miR-182 expression. Furthermore, we present miR-182 expression as a prognostic marker in cytogenetically high-risk acute myeloid leukemia patients. Our data demonstrate the importance of a controlled balance between C/EBP alpha and miR-182 for the maintenance of healthy granulopoiesis. Pracoviště Ústav molekulární genetiky Kontakt Nikol Škňouřilová, nikol.sknourilova@img.cas.cz, Tel.: 241 063 217 Rok sběru 2018
Počet záznamů: 1