Počet záznamů: 1
Different roles of CD4, CD8 and gamma delta T-lymphocytes in naive and vaccinated chickens during Salmonella Enteritidis infection
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SYSNO ASEP 0485640 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Different roles of CD4, CD8 and gamma delta T-lymphocytes in naive and vaccinated chickens during Salmonella Enteritidis infection Tvůrce(i) Sekelova, Z. (CZ)
Polanský, O. (CZ)
Štěpánová, H. (CZ)
Fedr, Radek (BFU-R) ORCID
Faldynová, M. (CZ)
Rychlík, I. (CZ)
Vlasatiková, L. (CZ)Celkový počet autorů 7 Číslo článku 1700073 Zdroj.dok. Proteomics. - : Wiley - ISSN 1615-9853
Roč. 17, č. 13-14 (2017)Poč.str. 9 s. Forma vydání Online - E Jazyk dok. eng - angličtina Země vyd. DE - Německo Klíč. slova dendritic cells ; activation ; cd28 Vědní obor RIV CE - Biochemie Obor OECD Biochemistry and molecular biology Institucionální podpora BFU-R - RVO:68081707 UT WOS 000405754500012 DOI 10.1002/pmic.201700073 Anotace Lymphocytes represent the key antigen-specific leukocyte subpopulation. Despite their importance in mounting an immune response, an unbiased description of proteins expressed by chicken lymphocytes has not been presented. In this study, we therefore intravenously infected chickens with Salmonella Enteritidis, sorted CD4, CD8 and gamma delta T-lymphocytes from the spleen by flow cytometry and determined the proteome of each population by LC-MS/MS. CD4 T-lymphocyte characteristic proteins included ubiquitin SUMO-like domain and BAR domain containing proteins. CD8 T-lymphocyte specific proteins were characterized by purine ribonucleoside triphosphate binding and were involved in cell differentiation, cell activation and regulation of programmed cell death. gamma delta T-lymphocyte specific proteins exhibited enrichment of small GTPase of Rab type and GTP binding. Following infection, inducible proteins in CD4 lymphocytes included ribosomal proteins and downregulated proteins localized to the lysosome. CD8 T-lymphocytes induced MCM complex proteins, proteins required for DNA replication and machinery for protein processing in the endoplasmic reticulum. Proteins inducible in gamma delta T-lymphocytes belonged to immune system response, oxidative phosphorylation and the spliceosome. In this study, we predicted the likely events in lymphocyte response to systemic bacterial infection and identified proteins which can be used as markers specific for each lymphocyte subpopulation. Pracoviště Biofyzikální ústav Kontakt Jana Poláková, polakova@ibp.cz, Tel.: 541 517 244 Rok sběru 2018
Počet záznamů: 1