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Identification and Characterization of Phospholipids with Very Long Chain Fatty Acids in Brewer's Yeast
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SYSNO ASEP 0482844 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Identification and Characterization of Phospholipids with Very Long Chain Fatty Acids in Brewer's Yeast Tvůrce(i) Řezanka, Tomáš (MBU-M) ORCID
Kolouchová, I. (CZ)
Gharwalová, L. (CZ)
Palyzová, Andrea (MBU-M) RID, ORCID
Sigler, Karel (MBU-M) RIDZdroj.dok. Lipids. - : Wiley - ISSN 0024-4201
Roč. 52, č. 12 (2017), s. 1007-1017Poč.str. 11 s. Jazyk dok. eng - angličtina Země vyd. US - Spojené státy americké Klíč. slova Yeast ; Very long chain fatty acids ; Negative electrospray ionization Vědní obor RIV EE - Mikrobiologie, virologie Obor OECD Microbiology CEP GA17-00027S GA ČR - Grantová agentura ČR Institucionální podpora MBU-M - RVO:61388971 UT WOS 000415878300005 EID SCOPUS 85029459669 DOI 10.1007/s11745-017-4294-6 Anotace Yeast lipids and fatty acids (FA) were analyzed in Saccharomyces pastorianus from seven breweries and in the dietary yeast supplement Pangamin. GC-MS identified more than 30 FA, half of which were very-long chain fatty acids (VLCFA) with hydrocarbon chain lengths of aeyen22 C atoms. Positional isomers omega-9 and omega-7 were identified in FA with C18-C28 even-numbered alkyl chains. The most abundant omega-7 isomer was cis-vaccenic acid. The structure of monounsaturated FA was proved by dimethyl disulfide adducts (position of double bonds and cis geometric configuration) and by GC-MS of pyridyl carbinol esters. Ultra-high performance liquid chromatography-tandem mass spectrometry with negative electrospray ionization identified the phospholipids phosphatidylethanolamine, phosphatidylinositol and phosphatidylcholine, with more than 150 molecular species. Wild-type unmutated brewer's yeast strains conventionally used for the manufacture of food supplements were found to contain VLCFA. Pracoviště Mikrobiologický ústav Kontakt Eliška Spurná, eliska.spurna@biomed.cas.cz, Tel.: 241 062 231 Rok sběru 2018
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