Počet záznamů: 1  

Extensive gain and loss of photosystem I subunits in chromerid algae, photosynthetic relatives of apicomplexans

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    SYSNO ASEP0480819
    Druh ASEPJ - Článek v odborném periodiku
    Zařazení RIVJ - Článek v odborném periodiku
    Poddruh JČlánek ve WOS
    NázevExtensive gain and loss of photosystem I subunits in chromerid algae, photosynthetic relatives of apicomplexans
    Tvůrce(i) Sobotka, Roman (MBU-M) RID, ORCID
    Esson, H.J. (CZ)
    Koník, Peter (MBU-M)
    Trsková, Eliška (MBU-M)
    Moravcová, Lenka (MBU-M)
    Horák, A. (CZ)
    Dufková, P. (CZ)
    Oborník, Miroslav (MBU-M) RID
    Číslo článku13214
    Zdroj.dok.Scientific Reports - ISSN 2045-2322
    Roč. 7, OCT 16 (2017), s. 1-13
    Poč.str.13 s.
    Jazyk dok.eng - angličtina
    Země vyd.GB - Velká Británie
    Klíč. slovaCYTOCHROME B(6)F COMPLEX ; WATER-WATER CYCLE ; LIGHT-HARVESTING ANTENNA
    Vědní obor RIVEE - Mikrobiologie, virologie
    Obor OECDMicrobiology
    CEPGBP501/12/G055 GA ČR - Grantová agentura ČR
    LO1416 GA MŠk - Ministerstvo školství, mládeže a tělovýchovy
    EE2.3.30.0059 GA MŠk - Ministerstvo školství, mládeže a tělovýchovy
    Institucionální podporaMBU-M - RVO:61388971
    UT WOS000413048000025
    EID SCOPUS85031790065
    DOI10.1038/s41598-017-13575-x
    AnotaceIn oxygenic photosynthesis the initial photochemical processes are carried out by photosystem I (PSI) and II (PSII). Although subunit composition varies between cyanobacterial and plastid photosystems, the core structures of PSI and PSII are conserved throughout photosynthetic eukaryotes. So far, the photosynthetic complexes have been characterised in only a small number of organisms. We performed in silico and biochemical studies to explore the organization and evolution of the photosynthetic apparatus in the chromerids Chromera velia and Vitrella brassicaformis, autotrophic relatives of apicomplexans. We catalogued the presence and location of genes coding for conserved subunits of the photosystems as well as cytochrome b(6)f and ATP synthase in chromerids and other phototrophs and performed a phylogenetic analysis. We then characterised the photosynthetic complexes of Chromera and Vitrella using 2D gels combined with mass-spectrometry and further analysed the purified Chromera PSI. Our data suggest that the photosynthetic apparatus of chromerids underwent unique structural changes. Both photosystems (as well as cytochrome b(6)f and ATP synthase) lost several canonical subunits, while PSI gained one superoxide dismutase (Vitrella) or two superoxide dismutases and several unknown proteins (Chromera) as new regular subunits. We discuss these results in light of the extraordinarily efficient photosynthetic processes described in Chromera.
    PracovištěMikrobiologický ústav
    KontaktEliška Spurná, eliska.spurna@biomed.cas.cz, Tel.: 241 062 231
    Rok sběru2018
Počet záznamů: 1