Počet záznamů: 1
Extensive gain and loss of photosystem I subunits in chromerid algae, photosynthetic relatives of apicomplexans
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SYSNO ASEP 0480819 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Extensive gain and loss of photosystem I subunits in chromerid algae, photosynthetic relatives of apicomplexans Tvůrce(i) Sobotka, Roman (MBU-M) RID, ORCID
Esson, H.J. (CZ)
Koník, Peter (MBU-M)
Trsková, Eliška (MBU-M)
Moravcová, Lenka (MBU-M)
Horák, A. (CZ)
Dufková, P. (CZ)
Oborník, Miroslav (MBU-M) RIDČíslo článku 13214 Zdroj.dok. Scientific Reports. - : Nature Publishing Group - ISSN 2045-2322
Roč. 7, OCT 16 (2017), s. 1-13Poč.str. 13 s. Jazyk dok. eng - angličtina Země vyd. GB - Velká Británie Klíč. slova CYTOCHROME B(6)F COMPLEX ; WATER-WATER CYCLE ; LIGHT-HARVESTING ANTENNA Vědní obor RIV EE - Mikrobiologie, virologie Obor OECD Microbiology CEP GBP501/12/G055 GA ČR - Grantová agentura ČR LO1416 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy EE2.3.30.0059 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy Institucionální podpora MBU-M - RVO:61388971 UT WOS 000413048000025 EID SCOPUS 85031790065 DOI 10.1038/s41598-017-13575-x Anotace In oxygenic photosynthesis the initial photochemical processes are carried out by photosystem I (PSI) and II (PSII). Although subunit composition varies between cyanobacterial and plastid photosystems, the core structures of PSI and PSII are conserved throughout photosynthetic eukaryotes. So far, the photosynthetic complexes have been characterised in only a small number of organisms. We performed in silico and biochemical studies to explore the organization and evolution of the photosynthetic apparatus in the chromerids Chromera velia and Vitrella brassicaformis, autotrophic relatives of apicomplexans. We catalogued the presence and location of genes coding for conserved subunits of the photosystems as well as cytochrome b(6)f and ATP synthase in chromerids and other phototrophs and performed a phylogenetic analysis. We then characterised the photosynthetic complexes of Chromera and Vitrella using 2D gels combined with mass-spectrometry and further analysed the purified Chromera PSI. Our data suggest that the photosynthetic apparatus of chromerids underwent unique structural changes. Both photosystems (as well as cytochrome b(6)f and ATP synthase) lost several canonical subunits, while PSI gained one superoxide dismutase (Vitrella) or two superoxide dismutases and several unknown proteins (Chromera) as new regular subunits. We discuss these results in light of the extraordinarily efficient photosynthetic processes described in Chromera. Pracoviště Mikrobiologický ústav Kontakt Eliška Spurná, eliska.spurna@biomed.cas.cz, Tel.: 241 062 231 Rok sběru 2018
Počet záznamů: 1