Počet záznamů: 1
Phenothiazine-linked nucleosides and nucleotides for redox labelling of DNA
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SYSNO ASEP 0479760 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Phenothiazine-linked nucleosides and nucleotides for redox labelling of DNA Tvůrce(i) Simonova, Anna (UOCHB-X)
Havran, Luděk (BFU-R) RID, ORCID
Pohl, Radek (UOCHB-X) RID, ORCID
Fojta, Miroslav (BFU-R) RID, ORCID
Hocek, Michal (UOCHB-X) RID, ORCIDZdroj.dok. Organic & Biomolecular Chemistry. - : Royal Society of Chemistry - ISSN 1477-0520
Roč. 15, č. 33 (2017), s. 6984-6996Poč.str. 13 s. Jazyk dok. eng - angličtina Země vyd. GB - Velká Británie Klíč. slova electrochemical detection ; primer extension ; 2'-deoxyribonucleoside triphosphates Vědní obor RIV CC - Organická chemie Obor OECD Organic chemistry CEP GBP206/12/G151 GA ČR - Grantová agentura ČR Institucionální podpora UOCHB-X - RVO:61388963 ; BFU-R - RVO:68081707 UT WOS 000411448200017 EID SCOPUS 85028298656 DOI 10.1039/c7ob01439b Anotace Nucleosides and 2'-deoxyribonucleoside triphosphates (dNTPs) bearing phenothiazine (PT) attached to a nucleobase (cytosine or 7-deazaadenine) either directly or through an acetylene linker were prepared through Suzuki or Sonogashira cross-coupling and triphosphorylation, and were studied as building blocks for polymerase construction of modified DNA. The directly PT-substituted dNTPs were better substrates for polymerases than the alkyne-linked dNTPs but all of them were used in enzymatic synthesis of DNA using primer extension, nicking enzyme amplification, PCR or 3'-tail labelling by terminal deoxynucleotidyl transferase. The phenothiazine served as an oxidizable redox label (giving two analytically useful signals of oxidation on electrode) for nucleosides and DNA and was also used in orthogonal combination with previously developed benzofurazane or nitrophenyl labels for redox coding of DNA bases. Therefore, the title PT-linked dNTPs are useful additions to the portfolio of nucleotides for enzymatic synthesis of redox-labelled DNA for electrochemical analysis. Pracoviště Ústav organické chemie a biochemie Kontakt asep@uochb.cas.cz ; Kateřina Šperková, Tel.: 232 002 584 ; Viktorie Chládková, Tel.: 232 002 434 Rok sběru 2018 Elektronická adresa http://pubs.rsc.org/en/content/articlehtml/2017/ob/c7ob01439b
Počet záznamů: 1