Počet záznamů: 1
Molecular MR imaging of fibrosis in a mouse model of pancreatic cancer
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SYSNO ASEP 0479455 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Molecular MR imaging of fibrosis in a mouse model of pancreatic cancer Tvůrce(i) Polášek, Miloslav (UOCHB-X) ORCID
Yang, Y. (US)
Schühle, D. T. (US)
Yaseen, M. A. (US)
Kim, Y. R. (US)
Sung, Y. S. (US)
Guimaraes, A. R. (US)
Caravan, P. (US)Číslo článku 8114 Zdroj.dok. Scientific Reports. - : Nature Publishing Group - ISSN 2045-2322
Roč. 7, Aug 14 (2017)Poč.str. 10 s. Jazyk dok. eng - angličtina Země vyd. GB - Velká Británie Klíč. slova fibrosis ; molecular imaging ; pancreatic cancer Vědní obor RIV FD - Onkologie a hematologie Obor OECD Oncology Institucionální podpora UOCHB-X - RVO:61388963 UT WOS 000407560600026 EID SCOPUS 85027456007 DOI 10.1038/s41598-017-08838-6 Anotace Fibrosis with excessive amounts of type I collagen is a hallmark of many solid tumours, and fibrosis is a promising target in cancer therapy, but tools for its non-invasive quantification are missing. Here we used magnetic resonance imaging with a gadolinium-based probe targeted to type I collagen (EP3533) to image and quantify fibrosis in pancreatic ductal adenocarcinoma. An orthotopic syngeneic mouse model resulted in tumours with 2.3-fold higher collagen level compared to healthy pancreas. Animals were scanned at 4.7T before, during and up to 60 min after i.v. injection of EP-3533, or of its non-binding isomer EP-3612. Ex-vivo quantification of gadolinium showed significantly higher uptake of EP-3533 compared to EP-3612 in tumours, but not in surrounding tissue (blood, muscle). Uptake of EP-3533 visualized in T1-weighted MRI correlated well with spatial distribution of collagen determined by second harmonic generation imaging. Differences in the tumour pharmacokinetic profiles of EP-3533 and EP-3612 were utilized to distinguish specific binding to tumour collagen from non-specific uptake. A model-free pharmacokinetic measurement based on area under the curve was identified as a robust imaging biomarker of fibrosis. Collagen-targeted molecular MRI with EP-3533 represents a new tool for non-invasive visualization and quantification of fibrosis in tumour tissue. Pracoviště Ústav organické chemie a biochemie Kontakt asep@uochb.cas.cz ; Kateřina Šperková, Tel.: 232 002 584 ; Viktorie Chládková, Tel.: 232 002 434 Rok sběru 2018 Elektronická adresa https://www.nature.com/articles/s41598-017-08838-6
Počet záznamů: 1