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Genomic Analysis of the Evolution of Phototrophy among Haloalkaliphilic Rhodobacterales
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SYSNO ASEP 0479403 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Genomic Analysis of the Evolution of Phototrophy among Haloalkaliphilic Rhodobacterales Tvůrce(i) Kopejtka, Karel (MBU-M) ORCID, RID
Tomasch, J. (DE)
Zeng, Y. (DK)
Tichý, Martin (MBU-M) RID
Sorokin, D. Y. (RU)
Koblížek, Michal (MBU-M) RID, ORCIDZdroj.dok. Genome Biology and Evolution. - : Oxford University Press - ISSN 1759-6653
Roč. 9, č. 7 (2017), s. 1950-1962Poč.str. 13 s. Jazyk dok. eng - angličtina Země vyd. GB - Velká Británie Klíč. slova Anoxygenic photosynthesis ; bacteriochlorophyll ; horizontal gene transfer Vědní obor RIV EE - Mikrobiologie, virologie Obor OECD Microbiology CEP GBP501/12/G055 GA ČR - Grantová agentura ČR LO1416 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy Institucionální podpora MBU-M - RVO:61388971 UT WOS 000410676100010 EID SCOPUS 85028405079 DOI 10.1093/gbe/evx141 Anotace A characteristic feature of the order Rhodobacterales is the presence of a large number of photoautotrophic and photoheterotrophic species containing bacteriochlorophyll. Interestingly, these phototrophic species are phylogenetically mixed with chemotrophs. To better understand the origin of such variability, we sequenced the genomes of three closely related haloalkaliphilic species, differing in their phototrophic capacity and oxygen preference: the photoheterotrophic and facultatively anaerobic bacterium Rhodobaca barguzinensis, aerobic photoheterotroph Roseinatronobacter thiooxidans, and aerobic heterotrophic bacteriumNatronohydrobacter thiooxidans. These three haloalcaliphilic species are phylogenetically related and share many common characteristics with the Rhodobacter species, forming together the Rhodobacter-Rhodobaca (RR) group. A comparative genomic analysis showed close homology of photosynthetic proteins and similarity in photosynthesis gene organization among the investigated phototrophic RR species. On the other hand, Rhodobaca barguzinensis and Roseinatronobacter thiooxidans lack an inorganic carbon fixation pathway and outer light-harvesting genes. This documents the reduction of their photosynthetic machinery towards a mostly photoheterotrophic lifestyle. Moreover, both phototrophic species contain 5-aminolevulinate synthase (encoded by the hemAgene) incorporated into their photosynthesis gene clusters, which seems to be a commonfeature of all aerobic anoxygenic phototrophic Alphaproteobacteria. Interestingly, the chrR-rpoE (sigma24) operon, which is part of singlet oxygen defense in phototrophic species, was found in the heterotrophic strain Natronohydrobacter thiooxidans. This suggests that this organism evolved from a photoheterotrophic ancestor through the loss of its photosynthesis genes. The overall evolution of phototrophy among the haloalkaliphilic members of the RR group is discussed. Pracoviště Mikrobiologický ústav Kontakt Eliška Spurná, eliska.spurna@biomed.cas.cz, Tel.: 241 062 231 Rok sběru 2018
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