Počet záznamů: 1
Lipidomic analysis of two closely related strains of the microalga Parietochloris (Trebouxiophyceae, Chlorophyta)
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SYSNO ASEP 0477524 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Lipidomic analysis of two closely related strains of the microalga Parietochloris (Trebouxiophyceae, Chlorophyta) Tvůrce(i) Řezanka, Tomáš (MBU-M) ORCID
Nedbalová, Linda (BU-J) RID, ORCID
Lukavský, Jaromír (BU-J) RID, ORCID
Procházková, L. (CZ)
Sigler, Karel (MBU-M) RIDZdroj.dok. Algal Research-Biomass Biofuels and Bioproducts. - : Elsevier - ISSN 2211-9264
Roč. 25, JUL 2017 (2017), s. 473-482Poč.str. 10 s. Jazyk dok. eng - angličtina Země vyd. NL - Nizozemsko Klíč. slova Parietochloris ; Arachidonic acid ; Lipidomics Vědní obor RIV EE - Mikrobiologie, virologie Obor OECD Microbiology Vědní obor RIV – spolupráce Botanický ústav - Biotechnologie a bionika CEP GA17-00027S GA ČR - Grantová agentura ČR Institucionální podpora MBU-M - RVO:61388971 ; BU-J - RVO:67985939 UT WOS 000406305900049 EID SCOPUS 85021058937 DOI 10.1016/j.algal.2017.06.005 Anotace The analysis of ribosomal internal transcribed spacer 2 (ITS2) secondary structure and morphology of two strains of the green microalga Parietochloris (CCALA 1082 and 1084) confirmed that they represent one biological species. However, their fatty acid profiles showed slight differences, which was fully confirmed by a series of lipidomic analyses using both shotgun lipidomics and hydrophilic interaction liquid chromatography-tandem mass spectrometry with electrospray ionization. 26 classes of lipids were identified, including more than 430 molecular species of lipids. The analyses showed that the greatest differences are in three types of phospholipids -phosphatidylethanolamine, phosphatidylcholine and phosphatidylinositol. These phospholipids have different spatial configurations (cylindrical, conical, and conical inverted), especially for those molecular species containing at least one arachidonic acid. Based on the lipidomic analyses we were able to distinguish the two strains. This may be advantageous in cases when routine DNA analysis shows that two strains are nearly identical. Pracoviště Mikrobiologický ústav Kontakt Eliška Spurná, eliska.spurna@biomed.cas.cz, Tel.: 241 062 231 Rok sběru 2018
Počet záznamů: 1