Počet záznamů: 1
Osteogenic differentiation of 3D cultured mesenchymal stem cells induced by bioactive peptides
- 1.
SYSNO ASEP 0477250 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Osteogenic differentiation of 3D cultured mesenchymal stem cells induced by bioactive peptides Tvůrce(i) Lukášová, Věra (UEM-P)
Buzgo, M. (CZ)
Sovková, Věra (UEM-P) ORCID
Daňková, Jana (UEM-P)
Rampichová, Michala (UEM-P) RID, ORCID
Amler, Evžen (UEM-P) RIDZdroj.dok. Cell Proliferation - ISSN 0960-7722
Roč. 50, č. 4 (2017), e12357Poč.str. 12 s. Jazyk dok. eng - angličtina Země vyd. GB - Velká Británie Klíč. slova bone morphogenetic protein-2 ; marrow stromal cells ; osteoblastic differentiation Vědní obor RIV EB - Genetika a molekulární biologie Obor OECD Cell biology CEP GA15-15697S GA ČR - Grantová agentura ČR GA16-14758S GA ČR - Grantová agentura ČR LO1309 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy LO1508 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy Institucionální podpora UEM-P - RVO:68378041 UT WOS 000407139200013 EID SCOPUS 85024127452 DOI 10.1111/cpr.12357 Anotace Objectives: Bioactive peptides derived from receptor binding motifs of native proteins are a potent source of bioactive molecules that can induce signalling pathways. These peptides could substitute for osteogenesis promoting supplements. The work presented here compares three kinds of bioactive peptides derived from collagen III, bone morphogenetic protein 7 (BMP-7) and BMP-2 with their potential osteogenic activity on the model of porcine mesenchymal stem cells (pMSCs).
Materials and methods: pMSCs were cultured on electrospun polycaprolactone nanofibrous scaffolds with different concentrations of the bioactive peptides without addition of any osteogenic supplement. Analysis of pMSCs cultures included measurement of the metabolic activity and proliferation, immunofluorescence staining and also qPCR.
Results: Results showed no detrimental effect of the bioactive peptides to cultured pMSCs. Based on qPCR analysis, the bioactive peptides are specific for osteogenic differentiation with no detectable expression of collagen II. Our results further indicate that peptide derived from BMP-2 protein promoted the expression of mRNA for osteocalcin (OCN) and collagen I significantly compared to control groups and also supported deposition of OCN as observed by immunostaining method.
Conclusion: The data suggest that bioactive peptide with an amino acid sequence of KIPKASSVPTELSAISTLYL derived from BMP-2 protein was the most potent for triggering osteogenic differentiation of pMSCs.Pracoviště Ústav experimentální medicíny Kontakt Lenka Koželská, lenka.kozelska@iem.cas.cz, Tel.: 241 062 218, 296 442 218 Rok sběru 2018
Počet záznamů: 1