Počet záznamů: 1
Comparison of targeted proteomics approaches for detecting and quantifying proteins derived from human cancer tissues
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SYSNO ASEP 0475939 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Comparison of targeted proteomics approaches for detecting and quantifying proteins derived from human cancer tissues Tvůrce(i) Faktor, J. (CZ)
Suchá, Rita (UZFG-Y) ORCID
Páralová, V. (CZ)
Liu, Y. (CH)
Bouchal, P. (CZ)Číslo článku 1600323 Zdroj.dok. Proteomics. - : Wiley - ISSN 1615-9853
Roč. 17, č. 5 (2017)Poč.str. 6 s. Forma vydání Tištěná - P Jazyk dok. eng - angličtina Země vyd. DE - Německo Klíč. slova cancer ; MRM/SRM ; SWATH Vědní obor RIV EI - Biotechnologie a bionika Obor OECD Technologies involving identifying the functioning of DNA, proteins and enzymes and how they influence the onset of disease and maintenance of well-being (gene-based diagnostics and therapeutic interventions (pharmacogenomics, gene-based therapeutics) CEP LO1609 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy Institucionální podpora UZFG-Y - RVO:67985904 UT WOS 000397390800006 EID SCOPUS 85013414422 DOI 10.1002/pmic.201600323 Anotace Targeted mass spectrometry-based proteomics approaches enable the simultaneous and reproducible quantification of multiple protein analytes across numerous conditions in biology and clinical studies. These approaches involve e.g. selected reaction monitoring (SRM) typically conducted on a triple quadrupole mass spectrometer, its high-resolution variant named pseudo-SRM (p-SRM), carried out in a quadrupole coupled with an TOF analyzer (qTOF), and sequential window acquisition of all theoretical spektra (SWATH). Here we compared these methods in terms of signal-to-noise ratio (S/N), coefficient of variance (CV), fold change (FC), limit of detection and quantitation (LOD, LOQ). We have shown the highest S/N for p-SRM mode, followed by SRM and SWATH, demonstrating a trade-off between sensitivity and level of multiplexing for SRM, p-SRM, and SWATH. SRM was more sensitive than p-SRM based on determining their LOD and LOQ. Although SWATH has the worst S/N, it enables peptidemultiplexing with post-acquisition definition of the targets, leading to better proteome coverage. FC between breast tumors of different clinical-pathological characteristics were highly correlated (R-2>0.97) across three methods and consistent with the previous study on 96 tumor tissues. Our technical note presented here, therefore, confirmed that outputs of all the three methods were biologically relevant and highly applicable to cancer research. Pracoviště Ústav živočišné fyziologie a genetiky Kontakt Jana Zásmětová, knihovna@iapg.cas.cz, Tel.: 315 639 554 Rok sběru 2018
Počet záznamů: 1