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Ganoderma lucidum and oxidative DNA damage: the role cellular antioxidant system
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SYSNO ASEP 0475624 Druh ASEP C - Konferenční příspěvek (mezinárodní konf.) Zařazení RIV O - Ostatní Název Ganoderma lucidum and oxidative DNA damage: the role cellular antioxidant system Tvůrce(i) Opattová, Alena (UEM-P)
Kozics, K. (SK)
Čumová, Andrea (UEM-P)
Slíva, D. (CZ)
Vodenková, S. (US)
Vodička, Pavel (UEM-P) RIDZdroj.dok. Cellular signalling and cancer therapy. - Cavtat : EMBO Conference, 2016 Rozsah stran s. 172-172 Poč.str. 1 s. Akce Cellular signalling and cancer therapy Datum konání 27.05.2016 - 31.05.2016 Místo konání Cavtat Země HR - Chorvatsko Jazyk dok. eng - angličtina Země vyd. HR - Chorvatsko Klíč. slova colorectal cancer ; natural compound ; oxidative DNA damage ; DNA repair ; colorectal cell lines Vědní obor RIV EE - Mikrobiologie, virologie CEP LH13061 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy GA15-14789S GA ČR - Grantová agentura ČR NV15-27580A GA MZd - Ministerstvo zdravotnictví Institucionální podpora UEM-P - RVO:68378041 Anotace Reactive oxygen species (ROS) are a group of highly reactive molecules tightly controlled by cellular antioxidant system. Disturbance in the prooxidation–antioxidation homeostasis can lead to ROS accumulation and consequently to DNA damage, as well as apoptosis. Many natural compounds such as Ganoderma lucidum (GLC) possess anticancer activities with the generation of ROS. Because the cancer cells are most sensitive to oxidative DNA damage as non-cancerous cells, oxidative damage is a potential target to enhance the activity of anticancer treatment by natural compounds which may lead to selective cancer cell death.
The aim of our study was to define effect of GLC extracts on cellular antioxidant system, oxidative DNA damage in colorectal cell lines (HTC116, HCT116-/-, HT29). Our results showed that 24 hrs GLC treatment (0,5mg/ml) inhibits activity of SOD1 (25%, p<0.01) in HCT116 as well as enzymatic activity of GpX (20%, p<0.01), followed by abnormal reactive oxygen species accumulation. Moreover, GLC significantly decreased a level of nuclear respiratory factor1 (NRF1), transcription factor critical for expression of antioxidant response dependent genes. The specific oxidative DNA damage increased (x%, p<0.05) after GLC treatment (0.5mg/ml, p<0.05), whereas the specific DNA repair process was inhibited. Finally, this led to decreased HCT116 survival (25%, p<0.05).
Our results clearly suggest that GLC extract strongly decrease activity of cellular antioxidant system and lead oxidative DNA damage and cell death in colorectal cancer cell lines. This indicates that natural compounds with prooxidant activity are potential target for selective improvement of anti-cancer treatment.
Pracoviště Ústav experimentální medicíny Kontakt Lenka Koželská, lenka.kozelska@iem.cas.cz, Tel.: 241 062 218, 296 442 218 Rok sběru 2017
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