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Adipokinetic hormones control amylase activity in the cockroach (Periplaneta americana) gut
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SYSNO ASEP 0475314 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Adipokinetic hormones control amylase activity in the cockroach (Periplaneta americana) gut Tvůrce(i) Bodláková, K. (CZ)
Jedlička, Pavel (UOCHB-X) ORCID, RID
Kodrík, Dalibor (BC-A) RID, ORCIDZdroj.dok. Insect Science. - : Wiley - ISSN 1672-9609
Roč. 24, č. 2 (2017), s. 259-269Poč.str. 11 s. Jazyk dok. eng - angličtina Země vyd. GB - Velká Británie Klíč. slova AKH ; AKH receptor ; amylase ; enzyme ; gene expression ; midgut Vědní obor RIV ED - Fyziologie Obor OECD Entomology CEP GA14-07172S GA ČR - Grantová agentura ČR Institucionální podpora UOCHB-X - RVO:61388963 ; BC-A - RVO:60077344 UT WOS 000398603200008 EID SCOPUS 84962641165 DOI 10.1111/1744-7917.12314 Anotace This study examined the biochemical characteristics of alpha-amylase and hormonal (adipokinetic hormone: AKH) stimulation of alpha-amylase activity in the cockroach (Periplaneta americana) midgut. We applied two AKHs in vivo and in vitro, then measured resultant amylase activity and gene expression, as well as the expression of AKH receptor (AKHR). The results revealed that optimal amylase activity is characterized by the following: pH: 5.7, temperature: 38.4 degrees C, K-m (Michaelis-Menten constant): 2.54 mg starch/mL, and V-max (maximum reaction velocity): 0.185 mu mol maltose/mL/min. In vivo application of AKHs resulted in significant increase of amylase activity: by two-fold in the gastric caeca and 4-7 fold in the rest of the midgut. In vitro experiments supported results seen in vivo: a 24-h incubation with the hormones resulted in the increase of amylase activity by 1.4 times in the caeca and 4-9 times in the midgut. Further, gene expression analyses reveal that AKHR is expressed in both the caeca and the rest of the midgut, although expression levels in the former were 23 times higher than levels in the latter. A similar pattern was found for the amylase (AMY) gene. Hormonal treatment did not affect the expression of either gene. This study is the first to provide evidence indicating direct AKH stimulation of digestive enzyme activity in the insect midgut, supported by specific AKHR gene expression in this organ. Pracoviště Ústav organické chemie a biochemie Kontakt asep@uochb.cas.cz ; Kateřina Šperková, Tel.: 232 002 584 ; Viktorie Chládková, Tel.: 232 002 434 Rok sběru 2018
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