Počet záznamů: 1

Distributions of therapeutically promising neurosteroids in cellular membranes

SYSNO ASEP	0475164
Druh ASEP	J - Článek v odborném periodiku
Zařazení RIV	J - Článek v odborném periodiku
Poddruh J	Článek ve WOS
Název	Distributions of therapeutically promising neurosteroids in cellular membranes
Tvůrce(i)	Riedlová, Kamila (UOCHB-X) Nekardová, Michaela (UOCHB-X)_RID Kačer, P. (CZ) Syslová, K. (CZ) Vazdar, M. (HR) Jungwirth, Pavel (UOCHB-X)_{RID, ORCID} Kudová, Eva (UOCHB-X)_{RID, ORCID} Cwiklik, Lukasz (UFCH-W)_{RID, ORCID}
Zdroj.dok.	Chemistry and Physics of Lipids. - : Elsevier - ISSN 0009-3084 Roč. 203, Mar (2017), s. 78-86
Poč.str.	9 s.
Jazyk dok.	eng - angličtina
Země vyd.	IE - Irsko
Klíč. slova	steroids ; N-methyl-D-aspartate receptors ; membranes ; molecular dynamics ; chromatography
Vědní obor RIV	CF - Fyzikální chemie a teoretická chemie
Obor OECD	Physical chemistry
Vědní obor RIV – spolupráce	Ústav fyzikální chemie J.Heyrovského - Fyzikální chemie a teoretická chemie
CEP	TE01020028 GA TA ČR - Technologická agentura ČR
	GAP303/12/1464 GA ČR - Grantová agentura ČR
	GA15-14292S GA ČR - Grantová agentura ČR
	GBP208/12/G016 GA ČR - Grantová agentura ČR
Institucionální podpora	UOCHB-X - RVO:61388963 ; UFCH-W - RVO:61388955
UT WOS	000395725500009
EID SCOPUS	85010992297
DOI	10.1016/j.chemphyslip.2016.12.004
Anotace	Interactions of two neurosteroids, inhibiting membrane-bound N-Methyl-D-aspartate receptors, with phospholipid membranes are studied. Namely, endogenous pregnanolone sulfate is compared with pregnanolone glutamate, the latter being a novel synthetic steroidal inhibitor of these receptors with potential pharmaceutical use. Molecular-level details of steroid-phospholipid membranes interactions are scrutinized employing molecular dynamics simulations supported by quantum chemical calculations to assess steroid lipophilicity. Moreover, permeability of both species across membranes is experimentally evaluated by immobilized artificial membrane chromatography. We demonstrate that while there is no significant difference of lipophilicity and membrane permeability between the two steroids, they differ significantly regarding detailed localization in phospholipid membranes. The bulky glutamate moiety of pregnanolone glutamate is flexible and well exposed to the water phase while the sulfate group of pregnanolone sulfate is hidden in the membrane headgroup region. This dissimilarity of behavior in membranes can potentially account for the observed different activities of the two steroids toward membrane-bound N-Methyl-D-aspartate receptors.
Pracoviště	Ústav organické chemie a biochemie
Kontakt	asep@uochb.cas.cz ; Kateřina Šperková, Tel.: 232 002 584 ; Viktorie Chládková, Tel.: 232 002 434
Rok sběru	2018

Počet záznamů: 1