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Lipid and carotenoid cooperation-driven adaptation to light and temperature stress in Synechocystis sp. PCC6803
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SYSNO ASEP 0475130 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Lipid and carotenoid cooperation-driven adaptation to light and temperature stress in Synechocystis sp. PCC6803 Tvůrce(i) Zakar, T. (HU)
Herman, E. (HU)
Vajravel, S. (HU)
Kovács, L. (HU)
Knoppová, Jana (MBU-M) RID
Komenda, Josef (MBU-M) RID, ORCID
Domonkos, I. (HU)
Kis, M. (HU)
Gombos, Z. (HU)
Laczko-Dobos, H. (HU)Zdroj.dok. Biochimica Et Biophysica Acta-Bioenergetics. - : Elsevier - ISSN 0005-2728
Roč. 1858, č. 5 (2017), s. 337-350Poč.str. 14 s. Jazyk dok. eng - angličtina Země vyd. NL - Nizozemsko Klíč. slova Lipid-carotenoid-protein interactions ; Lipid remodeling ; Xanthophylls Vědní obor RIV EE - Mikrobiologie, virologie Obor OECD Microbiology CEP LM2015055 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy LO1416 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy Institucionální podpora MBU-M - RVO:61388971 UT WOS 000401675700001 DOI 10.1016/j.bbabio.2017.02.002 Anotace Polyunsaturated lipids are important components of photosynthetic membranes. Xanthophylls are the main photoprotective agents, can assist in protection against light stress, and are crucial in the recovery from photoinhibition. We generated the xanthophyll- and polyunsaturated lipid-deficient ROAD mutant of Synechocystis sp. PCC6803 (Synechocystis) in order to study the little-known cooperative effects of lipids and carotenoids (Cars). Electron microscopic investigations confirmed that in the absence of xanthophylls the S-layer of the cellular envelope is missing. In wild-type (WT) cells, as well as the xanthophyll-less (RO), polyunsaturated lipid-less (AD), and the newly constructed ROAD mutants the lipid and Car compositions were determined by MS and HPLC, respectively. We found that, relative to the WT, the lipid composition of the mutants was remodeled and the Car content changed accordingly. In the mutants the ratio of non-bilayer-forming (NBL) to bilayer-forming (BL) lipids was found considerably lower. Xanthophyll to beta-carotene ratio increased in the AD mutant. In vitro and in vivo methods demonstrated that saturated, monounsaturated lipids and xanthophylls may stabilize the trimerization of Photosystem I (PSI). Fluorescence induction and oxygen-evolving activity measurements revealed increased light sensitivity of RO cells compared to those of the WT. ROAD showed a robust increase in light susceptibility and reduced recovery capability, especially at moderate low (ML) and moderate high (MH) temperatures, indicating a cooperative effect of xanthophylls and polyunsaturated lipids. We suggest that both lipid unsaturation and xanthophylls are required for providing the proper structure and functioning of the membrane environment that protects against light and temperature stress. Pracoviště Mikrobiologický ústav Kontakt Eliška Spurná, eliska.spurna@biomed.cas.cz, Tel.: 241 062 231 Rok sběru 2018
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