Počet záznamů: 1  

Magnetically modified bacterial cellulose: A promising carrier for immobilization of affinity ligands, enzymes, and cells

  1. 1.
    SYSNO ASEP0474812
    Druh ASEPJ - Článek v odborném periodiku
    Zařazení RIVJ - Článek v odborném periodiku
    Poddruh JČlánek ve WOS
    NázevMagnetically modified bacterial cellulose: A promising carrier for immobilization of affinity ligands, enzymes, and cells
    Tvůrce(i) Baldíková, E. (CZ)
    Pospíšková, K. (CZ)
    Ladakis, D. (GR)
    Kookos, I.K. (GR)
    Koutinas, A.A. (GR)
    Šafaříková, Miroslava (BC-A) RID
    Šafařík, Ivo (BC-A) RID, ORCID
    Zdroj.dok.Materials Science & Engineering C-Materials for Biological Applications. - : Elsevier - ISSN 0928-4931
    Roč. 71, February (2017), s. 214-221
    Poč.str.8 s.
    Jazyk dok.eng - angličtina
    Země vyd.NL - Nizozemsko
    Klíč. slovabacterial cellulose ; Komagataeibacter sucrofermentans ; copper phthalocyanine ; crystal violet ; yeast cells ; trypsin
    Vědní obor RIVEI - Biotechnologie a bionika
    Obor OECDBioproducts (products that are manufactured using biological material as feedstock) biomaterials, bioplastics, biofuels, bioderived bulk and fine chemicals, bio-derived novel materials
    Institucionální podporaBC-A - RVO:60077344
    UT WOS000390967200027
    EID SCOPUS84991730101
    DOI https://doi.org/10.1016/j.msec.2016.10.009
    AnotaceBacterial cellulose (BC) produced by Komagataeibacter sucrofermentans was magnetically modified using perchloric acid stabilized magnetic fluid. Magnetic bacterial cellulose (MBC) was used as a carrier for the immobilization of affinity ligands, enzymes and cells. MBC with immobilized reactive copper phthalocyanine dye was an efficient adsorbent for crystal violet removal, the maximum adsorption capacity was 388 mg/g. Kinetic and thermodynamic parameters were also determined. Model biocatalysts, namely bovine pancreas trypsin and Saccharomyces cerevisiae cells were immobilized on MBC using several strategies including adsorption with subsequent cross-linking with glutaraldehyde and covalent binding on previously activated MBC using sodium periodate or 1,4-butanediol diglycidyl ether. Immobilized yeast cells retained approximately 90% of their initial activity after 6 repeated cycles of sucrose solution hydrolysis. Trypsin covalently bound after MBC periodate activation was very stable during operational stability testing, it could be repeatedly used for ten cycles of low molecular weight substrate hydrolysis without loss of its initial activity.
    PracovištěBiologické centrum (od r. 2006)
    KontaktDana Hypšová, eje@eje.cz, Tel.: 387 775 214
    Rok sběru2018
Počet záznamů: 1  

  Tyto stránky využívají soubory cookies, které usnadňují jejich prohlížení. Další informace o tom jak používáme cookies.