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Magnetically modified bacterial cellulose: A promising carrier for immobilization of affinity ligands, enzymes, and cells
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SYSNO ASEP 0474812 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Magnetically modified bacterial cellulose: A promising carrier for immobilization of affinity ligands, enzymes, and cells Tvůrce(i) Baldíková, E. (CZ)
Pospíšková, K. (CZ)
Ladakis, D. (GR)
Kookos, I.K. (GR)
Koutinas, A.A. (GR)
Šafaříková, Miroslava (BC-A) RID
Šafařík, Ivo (BC-A) RID, ORCIDZdroj.dok. Materials Science & Engineering C-Materials for Biological Applications. - : Elsevier - ISSN 0928-4931
Roč. 71, February (2017), s. 214-221Poč.str. 8 s. Jazyk dok. eng - angličtina Země vyd. NL - Nizozemsko Klíč. slova bacterial cellulose ; Komagataeibacter sucrofermentans ; copper phthalocyanine ; crystal violet ; yeast cells ; trypsin Vědní obor RIV EI - Biotechnologie a bionika Obor OECD Bioproducts (products that are manufactured using biological material as feedstock) biomaterials, bioplastics, biofuels, bioderived bulk and fine chemicals, bio-derived novel materials Institucionální podpora BC-A - RVO:60077344 UT WOS 000390967200027 EID SCOPUS 84991730101 DOI https://doi.org/10.1016/j.msec.2016.10.009 Anotace Bacterial cellulose (BC) produced by Komagataeibacter sucrofermentans was magnetically modified using perchloric acid stabilized magnetic fluid. Magnetic bacterial cellulose (MBC) was used as a carrier for the immobilization of affinity ligands, enzymes and cells. MBC with immobilized reactive copper phthalocyanine dye was an efficient adsorbent for crystal violet removal, the maximum adsorption capacity was 388 mg/g. Kinetic and thermodynamic parameters were also determined. Model biocatalysts, namely bovine pancreas trypsin and Saccharomyces cerevisiae cells were immobilized on MBC using several strategies including adsorption with subsequent cross-linking with glutaraldehyde and covalent binding on previously activated MBC using sodium periodate or 1,4-butanediol diglycidyl ether. Immobilized yeast cells retained approximately 90% of their initial activity after 6 repeated cycles of sucrose solution hydrolysis. Trypsin covalently bound after MBC periodate activation was very stable during operational stability testing, it could be repeatedly used for ten cycles of low molecular weight substrate hydrolysis without loss of its initial activity. Pracoviště Biologické centrum (od r. 2006) Kontakt Dana Hypšová, eje@eje.cz, Tel.: 387 775 214 Rok sběru 2018
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