Počet záznamů: 1
PRC2 Represses Hormone-Induced Somatic Embryogenesis in Vegetative Tissue of Arabidopsis thaliana
- 1.
SYSNO ASEP 0474503 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název PRC2 Represses Hormone-Induced Somatic Embryogenesis in Vegetative Tissue of Arabidopsis thaliana Tvůrce(i) Mozgová, Iva (MBU-M)
Munoz-Viana, R. (SE)
Hennig, L. (SE)Číslo článku e1006562 Zdroj.dok. PLoS Genetics. - : Public Library of Science - ISSN 1553-7404
Roč. 13, č. 1 (2017), s. 1-27Poč.str. 27 s. Jazyk dok. eng - angličtina Země vyd. US - Spojené státy americké Klíč. slova PRC2 ; SE formation ; Arabidopsis thaliana Vědní obor RIV EE - Mikrobiologie, virologie Obor OECD Microbiology CEP GJ16-08423Y GA ČR - Grantová agentura ČR LO1416 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy Institucionální podpora MBU-M - RVO:61388971 UT WOS 000394147700033 EID SCOPUS 85011385367 DOI 10.1371/journal.pgen.1006562 Anotace Many plant cells can be reprogrammed into a pluripotent state that allows ectopic organ development. Inducing totipotent states to stimulate somatic embryo (SE) development is, however, challenging due to insufficient understanding of molecular barriers that prevent somatic cell dedifferentiation. Here we show that Polycomb repressive complex 2 (PRC2)-activity imposes a barrier to hormone-mediated transcriptional reprogramming towards somatic embryogenesis in vegetative tissue of Arabidopsis thaliana. We identify factors that enable SE development in PRC2-depleted shoot and root tissue and demonstrate that the establishment of embryogenic potential is marked by ectopic co-activation of crucial developmental regulators that specify shoot, root and embryo identity. Using inducible activation of PRC2 in PRC2-depleted cells, we demonstrate that transient reduction of PRC2 activity is sufficient for SE formation. We suggest that modulation of PRC2 activity in plant vegetative tissue combined with targeted activation of developmental pathways will open possibilities for novel approaches to cell reprogramming. Pracoviště Mikrobiologický ústav Kontakt Eliška Spurná, eliska.spurna@biomed.cas.cz, Tel.: 241 062 231 Rok sběru 2018
Počet záznamů: 1