Počet záznamů: 1
Differential Binding of Mitochondrial Transcripts by MRB8170 and MRB4160 Regulates Distinct Editing Fates of Mitochondrial mRNA in Trypanosomes
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SYSNO ASEP 0474329 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Differential Binding of Mitochondrial Transcripts by MRB8170 and MRB4160 Regulates Distinct Editing Fates of Mitochondrial mRNA in Trypanosomes Tvůrce(i) Dixit, Sameer (BC-A)
Mueller-McNicoll, M. (DE)
David, Vojtěch (BC-A)
Zarnack, K. (DE)
Ule, J. (GB)
Hashimi, Hassan (BC-A) RID, ORCID
Lukeš, Julius (BC-A) RID, ORCIDCelkový počet autorů 7 Číslo článku e02288-16 Zdroj.dok. mBio. - : American Society for Microbiology - ISSN 2161-2129
Roč. 8, č. 1 (2017)Poč.str. 15 s. Jazyk dok. eng - angličtina Země vyd. US - Spojené státy americké Klíč. slova guide RNA ; ribonucleoprotein complexes ; nucleotide resolution ; proteins ; DNA ; replication ; tbrgg2 ; subcomplexes ; translation Vědní obor RIV EB - Genetika a molekulární biologie Obor OECD Biochemistry and molecular biology CEP GA15-21974S GA ČR - Grantová agentura ČR Institucionální podpora BC-A - RVO:60077344 UT WOS 000395835000018 EID SCOPUS 85014843730 DOI 10.1128/mBio.02288-16 Anotace A dozen mRNAs are edited by multiple insertions and/or deletions of uridine residues in the mitochondrion of Trypanosoma brucei. Several protein complexes have been implicated in performing this type of RNA editing, including the mitochondrial RNA-binding complex 1 (MRB1). Two paralogous novel RNA-binding proteins, MRB8170 and MRB4160, are loosely associated with the core MRB1 complex. Their roles in RNA editing and effects on target mRNAs are so far not well understood. In this study, individual-nucleotide-resolution UV-cross-linking and affinity purification (iCLAP) revealed a preferential binding of both proteins to mitochondrial mRNAs, which was positively correlated with their extent of editing. Integrating additional in vivo and in vitro data, we propose that binding of MRB8170 and/or MRB4160 onto pre-mRNA marks it for the initiation of editing and that initial binding of both proteins may facilitate the recruitment of other components of the RNA editing/processing machinery to ensure efficient editing. Surprisingly, MRB8170 also binds never-edited mRNAs, suggesting that at least this paralog has an additional role outside RNA editing to shape the mitochondrial transcriptome. Pracoviště Biologické centrum (od r. 2006) Kontakt Dana Hypšová, eje@eje.cz, Tel.: 387 775 214 Rok sběru 2018
Počet záznamů: 1