Počet záznamů: 1
Effect of therapeutic concentration of lithium on live HEK293 cells, increase of Na+/K+-ATPase, change of overall protein composition and alteration of surface layer of plasma membrane
- 1.
SYSNO ASEP 0474133 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Effect of therapeutic concentration of lithium on live HEK293 cells, increase of Na+/K+-ATPase, change of overall protein composition and alteration of surface layer of plasma membrane Tvůrce(i) Vošahlíková, Miroslava (FGU-C) RID, ORCID, SAI
Ujčíková, Hana (FGU-C) RID, ORCID
Chernyavskiy, Oleksandr (FGU-C)
Brejchová, Jana (FGU-C) RID, ORCID
Roubalová, Lenka (FGU-C) RID, ORCID, SAI
Alda, M. (CZ)
Svoboda, Petr (FGU-C) RID, ORCIDZdroj.dok. Biochimica et Biophysica Acta-General Subjects. - : Elsevier - ISSN 0304-4165
Roč. 1861, č. 5 (2017), s. 1099-1112Poč.str. 14 s. Jazyk dok. eng - angličtina Země vyd. NL - Nizozemsko Klíč. slova lithium ; HEK293 cells ; Na+/K+-ATPase ; membrane biophysics ; proteomic analysis Vědní obor RIV CE - Biochemie Obor OECD Biochemistry and molecular biology CEP GAP207/12/0919 GA ČR - Grantová agentura ČR GA15-16605S GA ČR - Grantová agentura ČR GA17-07070S GA ČR - Grantová agentura ČR Institucionální podpora FGU-C - RVO:67985823 UT WOS 000403733500014 EID SCOPUS 85014325916 DOI 10.1016/j.bbagen.2017.02.011 Anotace The effect of long-term exposure of live cells to lithium cations (Li) was studied in HEK293 cells cultivated in the presence of 1 mM LiCl for 7 or 21 days. The alteration of Na+/K+-ATPase level, protein composition and biophysical state of plasma membrane was determined with the aim to characterize the physiological state of Li-treated cells. Na+/K+-ATPase level was determined by [3H]ouabain binding and immunoblot assays. Overall protein composition was determined by 2D electrophoresis followed by proteomic analysis by MALDI-TOF MS/MS and LFQ. Li interaction with plasma membrane was characterized by fluorescent probes DPH, TMA-DPH and Laurdan. Na+/K+-ATPase was increased in plasma membranes isolated from cells exposed to Li. Identification of Li-altered proteins by 2D electrophoresis, MALDI-TOF MS/MS and LFQ suggests a change of energy metabolism in mitochondria and cytosol and alteration of cell homeostasis of calcium. Measurement of Laurdan generalized polarization indicated a significant alteration of surface layer of isolated plasma membranes prepared from both types of Li-treated cells. Prolonged exposure of HEK293 cells to 1 mM LiCl results in up-regulation of Na+/K+-ATPase expression, reorganization of overall cellular metabolism and alteration of the surface layer/polar head-group region of isolated plasma membranes. Our findings demonstrate adaptation of live HEK293 cell metabolism to prolonged exposure to therapeutic concentration of Li manifested as up-regulation of Na+/K+-ATPase expression, alteration of protein composition and change of the surface layer of plasma membrane. Pracoviště Fyziologický ústav Kontakt Lucie Trajhanová, lucie.trajhanova@fgu.cas.cz, Tel.: 241 062 400 Rok sběru 2018
Počet záznamů: 1