Počet záznamů: 1
Aging does not compromise in vitro oscillation of the suprachiasmatic nuclei but makes it more vulnerable to constant light
- 1.
SYSNO ASEP 0473999 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Aging does not compromise in vitro oscillation of the suprachiasmatic nuclei but makes it more vulnerable to constant light Tvůrce(i) Polidarová, Lenka (FGU-C) RID, ORCID
Sládek, Martin (FGU-C) RID, ORCID, SAI
Novosadová, Zuzana (FGU-C) ORCID
Sumová, Alena (FGU-C) RID, ORCIDZdroj.dok. Chronobiology International - ISSN 0742-0528
Roč. 34, č. 1 (2017), s. 105-117Poč.str. 13 s. Jazyk dok. eng - angličtina Země vyd. US - Spojené státy americké Klíč. slova aging ; circadian clock ; constant light ; suprachiasmatic nuclei ; mPer2Luc mice Vědní obor RIV ED - Fyziologie Obor OECD Physiology (including cytology) CEP GA14-07711S GA ČR - Grantová agentura ČR Institucionální podpora FGU-C - RVO:67985823 UT WOS 000396728000009 EID SCOPUS 84992530912 DOI 10.1080/07420528.2016.1242491 Anotace Circadian regulation of behavior worsens with age, however, the mechanism behind this phenomenon is still poorly understood. Specifically, it is not clear to what extend the ability of the circadian clock in the suprachiasmatic nuclei (SCN) to generate the rhythm is affected by aging. This study aimed to ascertain the effect of aging on the functioning of the SCN of mPer2Luciferase mice under unnatural lighting conditions, such as constant light (LL). Under LL, which worsened the age-induced effect on behavioral rhythms, a marginal age-dependent effect on in vitro rhythmicity in explants containing the middle, but not the rostral/caudal, regions of the SCN was apparent, the proportion of mice in which middle-region SCN explants were completely arrhythmic or had an extremely long period (>30 h) was 47% in aged mice and 27% in adults. The results suggest that in some of the aged animals, LL may weaken the coupling among oscillators in specific sub-regions of the SCN, leaving other sub-regions better synchronized. In the standard light/dark cycle and in constant darkness, the SCN ability to produce bioluminescence rhythms in vitro was not compromised in aged mice although aging significantly affected their SCN-driven locomotor activity rhythms. Therefore, our results demonstrate that although age worsened the SCN output rhythm, the SCN molecular core clock mechanism itself was relatively resilient to aging in these same animals. The results suggest the involvement of pathways downstream of the core clock mechanism which are responsible for this phenomenon. Pracoviště Fyziologický ústav Kontakt Lucie Trajhanová, lucie.trajhanova@fgu.cas.cz, Tel.: 241 062 400 Rok sběru 2018
Počet záznamů: 1