Počet záznamů: 1
Phosphonium Carbosilane Dendrimers for Biomedical Applications – Synthesis, Characterization and Cytotoxicity Evaluation.
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SYSNO ASEP 0473749 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Phosphonium Carbosilane Dendrimers for Biomedical Applications – Synthesis, Characterization and Cytotoxicity Evaluation. Tvůrce(i) Strašák, Tomáš (UCHP-M) RID, ORCID, SAI
Malý, J. (CZ)
Wróbel, D. (CZ)
Malý, M. (CZ)
Herma, R. (CZ)
Čermák, Jan (UCHP-M) RID, ORCID, SAI
Müllerová, Monika (UCHP-M) RID, ORCID, SAI
Červenková Šťastná, Lucie (UCHP-M) RID, ORCID, SAI
Cuřínová, Petra (UCHP-M) RID, SAI, ORCIDZdroj.dok. RSC Advances. - : Royal Society of Chemistry - ISSN 2046-2069
Roč. 7, č. 30 (2017), s. 18724-18744Poč.str. 21 s. Jazyk dok. eng - angličtina Země vyd. GB - Velká Británie Klíč. slova modified ppi pendrimers ; molecular-dynamics ; gene delivery Vědní obor RIV CC - Organická chemie Obor OECD Organic chemistry Institucionální podpora UCHP-M - RVO:67985858 UT WOS 000399005500067 EID SCOPUS 85016408382 DOI https://doi.org/10.1039/c7ra01845b Anotace We report the synthesis and cytotoxicity evaluation of a completely new class of cationic carbosilane dendrimers functionalized with several different phosphonium peripheral groups and an ammonium functionalised one as a reference. The carbosilane dendrimers with NMe3, PMe3, P(Et2)2(CH2)3OH, PBu3, P(C6H4-OMe)3 and P(Ph)3 peripheral substituents were synthesized, thoroughly characterized and modelled by computer simulations. The cytotoxicities of the dendrimers were investigated in vitro on three model cell lines (B14, BRL and NRK cells) by MTT and CV assay methods. Generally, the cytotoxicities of PMe3 carbosilane dendrimers were similar or slightly lower when compared with NMe3 dendrimers. The substitution of methyl groups in PMe3 carbosilane dendrimers with more hydrophobic and bulky alkyl substituents (PBu3 and P(Et2)2(CH2)3OH dendrimers) resulted in an increase of cytotoxicity. The P(C6H4-OMe)3 dendrimer showed exceptionally low cytotoxicity across all cell lines or assay methods used. Generally, phosphonium carbosilane dendrimers could represent a valuable alternative to ammonium ones in gene therapy applications due to comparable or lower cytotoxicities, the presence of positive charge for nucleic acid electrostatic binding and in the cases of P(C6H4-OMe)3 and P(Ph)3 dendrimers high potential of mitochondrial targeting. Pracoviště Ústav chemických procesů Kontakt Eva Jirsová, jirsova@icpf.cas.cz, Tel.: 220 390 227 Rok sběru 2018
Počet záznamů: 1