Počet záznamů: 1  

Phosphonium Carbosilane Dendrimers for Biomedical Applications – Synthesis, Characterization and Cytotoxicity Evaluation.

  1. 1.
    SYSNO ASEP0473749
    Druh ASEPJ - Článek v odborném periodiku
    Zařazení RIVJ - Článek v odborném periodiku
    Poddruh JČlánek ve WOS
    NázevPhosphonium Carbosilane Dendrimers for Biomedical Applications – Synthesis, Characterization and Cytotoxicity Evaluation.
    Tvůrce(i) Strašák, Tomáš (UCHP-M) RID, ORCID, SAI
    Malý, J. (CZ)
    Wróbel, D. (CZ)
    Malý, M. (CZ)
    Herma, R. (CZ)
    Čermák, Jan (UCHP-M) RID, ORCID, SAI
    Müllerová, Monika (UCHP-M) RID, ORCID, SAI
    Červenková Šťastná, Lucie (UCHP-M) RID, ORCID, SAI
    Cuřínová, Petra (UCHP-M) RID, SAI, ORCID
    Zdroj.dok.RSC Advances. - : Royal Society of Chemistry
    Roč. 7, č. 30 (2017), s. 18724-18744
    Poč.str.21 s.
    Jazyk dok.eng - angličtina
    Země vyd.GB - Velká Británie
    Klíč. slovamodified ppi pendrimers ; molecular-dynamics ; gene delivery
    Vědní obor RIVCC - Organická chemie
    Obor OECDOrganic chemistry
    Institucionální podporaUCHP-M - RVO:67985858
    UT WOS000399005500067
    EID SCOPUS85016408382
    DOI10.1039/c7ra01845b
    AnotaceWe report the synthesis and cytotoxicity evaluation of a completely new class of cationic carbosilane dendrimers functionalized with several different phosphonium peripheral groups and an ammonium functionalised one as a reference. The carbosilane dendrimers with NMe3, PMe3, P(Et2)2(CH2)3OH, PBu3, P(C6H4-OMe)3 and P(Ph)3 peripheral substituents were synthesized, thoroughly characterized and modelled by computer simulations. The cytotoxicities of the dendrimers were investigated in vitro on three model cell lines (B14, BRL and NRK cells) by MTT and CV assay methods. Generally, the cytotoxicities of PMe3 carbosilane dendrimers were similar or slightly lower when compared with NMe3 dendrimers. The substitution of methyl groups in PMe3 carbosilane dendrimers with more hydrophobic and bulky alkyl substituents (PBu3 and P(Et2)2(CH2)3OH dendrimers) resulted in an increase of cytotoxicity. The P(C6H4-OMe)3 dendrimer showed exceptionally low cytotoxicity across all cell lines or assay methods used. Generally, phosphonium carbosilane dendrimers could represent a valuable alternative to ammonium ones in gene therapy applications due to comparable or lower cytotoxicities, the presence of positive charge for nucleic acid electrostatic binding and in the cases of P(C6H4-OMe)3 and P(Ph)3 dendrimers high potential of mitochondrial targeting.
    PracovištěÚstav chemických procesů
    KontaktEva Jirsová, jirsova@icpf.cas.cz, Tel.: 220 390 227
    Rok sběru2018
Počet záznamů: 1  

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