Počet záznamů: 1
Characterization of tetraspanin protein CD81 in mouse spermatozoa and bovine gametes
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SYSNO ASEP 0472835 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Characterization of tetraspanin protein CD81 in mouse spermatozoa and bovine gametes Tvůrce(i) Jankovičová, J. (SK)
Frolíková, Michaela (BTO-N) ORCID
Šebková, Nataša (BTO-N)
Simon, M. (SK)
Cupperova, P. (SK)
Lipcseyova, D. (SK)
Michalková, K. (SK)
Horovská, L. (SK)
Sedláček, Radislav (UMG-J) RID
Stopka, P. (CZ)
Antalíková, J. (SK)
Dvořáková-Hortová, Kateřina (BTO-N)Zdroj.dok. Reproduction. - : BioScientifica - ISSN 1470-1626
Roč. 152, č. 6 (2016), s. 785-793Poč.str. 14 s. Jazyk dok. eng - angličtina Země vyd. GB - Velká Británie Klíč. slova ACROSOME REACTION ; CD9-DEFICIENT MICE ; SPERM Vědní obor RIV EB - Genetika a molekulární biologie Vědní obor RIV – spolupráce Ústav molekulární genetiky - Genetika a molekulární biologie CEP ED1.1.00/02.0109 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy LM2011032 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy GA14-05547S GA ČR - Grantová agentura ČR Institucionální podpora BTO-N - RVO:86652036 ; UMG-J - RVO:68378050 UT WOS 000387771600025 EID SCOPUS 84996635642 DOI 10.1530/REP-16-0304 Anotace Sperm-egg interaction and fusion represent a key moment of fertilization. In mammals, it is not possible without the interaction of the tetraspanin superfamily proteins including CD81. A detailed immunohistochemical localization of CD81 was monitored in bovine oocytes during different maturation stages, as well as during early embryogenesis. In addition, characterization of CD81 was carried out in bovine and mouse sperm. In bovine eggs, CD81 was detected on the plasma membrane of the germinal vesicle, metaphase I and metaphase II oocytes. During fertilization, accumulation of CD81 molecules in the perivitelline space of fertilized oocytes, which appeared as vesicles associated with plasma membrane, was observed. In majority of bull-ejaculated sperm and caput, corpus and cauda epididymal sperm, as well as mouse cauda epididymal sperm, CD81 was found on the plasma membrane covering the apical acrosome. Although the process of capacitation did not influence the localization of CD81, it was lost from the surface of the acrosome-reacted spermatozoa in bull, in contrast to mouse sperm where there was a relocalization of the CD81 protein during acrosome reaction across the equatorial segment and later over the whole sperm head. The presented results highlight conservative unifying aspects of CD81 expression between cattle and mouse, together with mouse-specific traits in sperm CD81 behaviour, which emphasizes certain species-specific mechanisms of fertilization to be considered. Pracoviště Biotechnologický ústav Kontakt Monika Kopřivová, Monika.Koprivova@ibt.cas.cz, Tel.: 325 873 700 Rok sběru 2017
Počet záznamů: 1