Počet záznamů: 1
Identification and characterization of Xenopus tropicalis common progenitors of Sertoli and peritubular myoid cell lineages
- 1.
SYSNO ASEP 0472500 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Identification and characterization of Xenopus tropicalis common progenitors of Sertoli and peritubular myoid cell lineages Tvůrce(i) Tlapáková, T. (CZ)
Nguyen, T.M.X. (CZ)
Vegrichtova, M. (CZ)
Šídová, Monika (BTO-N) RID
Strnadova, K. (CZ)
Bláhová, M. (CZ)
Krylov, V. (CZ)Celkový počet autorů 7 Zdroj.dok. Biology Open. - : Company of Biologists - ISSN 2046-6390
Roč. 5, č. 9 (2016), s. 1275-1282Poč.str. 13 s. Jazyk dok. eng - angličtina Země vyd. GB - Velká Británie Klíč. slova Testicular somatic cells ; Xenopus tropicalis ; Migration potential Vědní obor RIV EB - Genetika a molekulární biologie CEP LK21305 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy ED1.1.00/02.0109 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy Institucionální podpora BTO-N - RVO:86652036 UT WOS 000384077300011 DOI 10.1242/bio.019265 Anotace The origin of somatic cell lineages during testicular development is controversial in mammals. Employing basal amphibian tetrapod Xenopus tropicalis we established a cell culture derived from testes of juvenile male. Expression analysis showed transcription of some pluripotency genes and Sertoli cell, peritubular myoid cell and mesenchymal cell markers. Transcription of germline-specific genes was downregulated. Immunocytochemistry revealed that a majority of cells express vimentin and co-express Sox9 and smooth muscle alpha-actin (Sma), indicating the existence of a common progenitor of Sertoli and peritubular myoid cell lineages. Microinjection of transgenic, red fluorescent protein (RFP)-positive somatic testicular cells into the peritoneal cavity of X. tropicalis tadpoles resulted in cell deposits in heart, pronephros and intestine, and later in a strong proliferation and formation of cell-to-cell net growing through the tadpole body. Immunohistochemistry analysis of transplanted tadpoles showed a strong expression of vimentin in RFP-positive cells. No co-localization of Sox9 and Sma signals was observed during the first three weeks indicating their dedifferentiation to migratory-active mesenchymal cells recently described in human testicular biopsies. Pracoviště Biotechnologický ústav Kontakt Monika Kopřivová, Monika.Koprivova@ibt.cas.cz, Tel.: 325 873 700 Rok sběru 2017
Počet záznamů: 1