Počet záznamů: 1
New Regulatory Roles of Galectin-3 in High-Affinity IgE Receptor Signaling
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SYSNO ASEP 0471873 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název New Regulatory Roles of Galectin-3 in High-Affinity IgE Receptor Signaling Tvůrce(i) Bambousková, Monika (UMG-J)
Polakovičová, Iva (UMG-J)
Hálová, Ivana (UMG-J) RID, ORCID
Goel, G. (US)
Dráberová, Lubica (UMG-J) RID
Bugajev, Viktor (UMG-J) RID
Doan, A. (US)
Utekal, Pavol (UMG-J)
Gardet, A. (US)
Xavier, R.J. (US)
Dráber, Petr (UMG-J) RIDCelkový počet autorů 11 Zdroj.dok. Molecular and Cellular Biology. - : American Society for Microbiology - ISSN 0270-7306
Roč. 36, č. 9 (2016), s. 1366-1382Poč.str. 17 s. Jazyk dok. eng - angličtina Země vyd. US - Spojené státy americké Klíč. slova fc-epsilon-ri ; t-cell-activation ; basophilic leukemia-cells ; mast-cell ; immunoglobulin-e ; tyrosine kinase ; lipid rafts ; monoclonal-antibody ; negative regulation ; mediator release Vědní obor RIV EB - Genetika a molekulární biologie CEP GBP302/12/G101 GA ČR - Grantová agentura ČR Institucionální podpora UMG-J - RVO:68378050 UT WOS 000374110900005 DOI 10.1128/MCB.00064-16 Anotace Aggregation of the high-affinity receptor for IgE (Fc epsilon RI) in mast cells initiates activation events that lead to degranulation and release of inflammatory mediators. To better understand the signaling pathways and genes involved in mast cell activation, we developed a high-throughput mast cell degranulation assay suitable for RNA interference experiments using lentivirus-based short hairpin RNA (shRNA) delivery. We tested 432 shRNAs specific for 144 selected genes for effects on Fc epsilon RI-mediated mast cell degranulation and identified 15 potential regulators. In further studies, we focused on galectin-3 (Gal3), identified in this study as a negative regulator of mast cell degranulation. Fc epsilon RI-activated cells with Gal3 knockdown exhibited upregulated tyrosine phosphorylation of spleen tyrosine kinase and several other signal transduction molecules and enhanced calcium response. We show that Gal3 promotes internalization of IgE-Fc epsilon RI complexes; this may be related to our finding that Gal3 is a positive regulator of Fc epsilon RI ubiquitination. Furthermore, we found that Gal3 facilitates mast cell adhesion and motility on fibronectin but negatively regulates antigen-induced chemotaxis. The combined data indicate that Gal3 is involved in both positive and negative regulation of Fc epsilon RI-mediated signaling events in mast cells. Pracoviště Ústav molekulární genetiky Kontakt Nikol Škňouřilová, nikol.sknourilova@img.cas.cz, Tel.: 241 063 217 Rok sběru 2017
Počet záznamů: 1