Počet záznamů: 1
The colonization of Bacillus thuringiensis strains in bryophytes
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SYSNO ASEP 0471433 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název The colonization of Bacillus thuringiensis strains in bryophytes Tvůrce(i) Lin, Q. (CN)
Zhu, P. (CN)
Carballar-Lejarazú, R. (US)
Gelbič, Ivan (BC-A) RID, ORCID
Guan, X. (CN)
Xu, L. (CN)
Zhang, L. (CN)Celkový počet autorů 7 Zdroj.dok. Turkish Journal of Biology - ISSN 1300-0152
Roč. 41, č. 1 (2017), s. 41-48Poč.str. 8 s. Jazyk dok. eng - angličtina Země vyd. TR - Turecko Klíč. slova Bacillus thuringiensis ; GFP ; plant colonization Vědní obor RIV EE - Mikrobiologie, virologie Obor OECD Microbiology Institucionální podpora BC-A - RVO:60077344 UT WOS 000394539800005 EID SCOPUS 85013436348 DOI 10.3906/biy-1510-16 Anotace In our previous study, several Bacillus thuringiensis (Bt) strains were isolated from bryophyte samples, indicating that bryophytes could serve as Bt reservoirs in the wild. SFR13 is a wild strain isolated from the bryophyta Physcomitrium japonicum. In order to understand its ecological properties, green fluorescent protein (GFP)-labelled SFR13 (SFR13GFP) was generated to evaluate the colonization capability in bryophytes, using dynamic tracing and cell counting to observe the process and patterns of colonization. Our results showed that genetic stability, growth curve dynamics, and insecticidal crystal production were not affected by GFP expression in Bt. Fluorescence microscopy was used to track the dynamic distribution of SFR13GFP. Distribution patterns showed that SFR13GFP can establish stable and long-term colonization in leaves and stems by the 26th day after inoculation. A better understanding of how Bt colonizes plants in the wild will not only result in increased knowledge of plant-microbe interactions but will also lead to a more successful and reliable use of bacterial inoculants. Pracoviště Biologické centrum (od r. 2006) Kontakt Dana Hypšová, eje@eje.cz, Tel.: 387 775 214 Rok sběru 2018 Elektronická adresa http://journals.tubitak.gov.tr/biology/issues/biy-17-41-1/biy-41-1-5-1510-16.pdf
Počet záznamů: 1