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Eukaryotic translation initiation factor 3 plays distinct roles at the mRNA entry and exit channels of the ribosomal preinitiation complex.
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SYSNO ASEP 0468167 Druh ASEP J - Článek v odborném periodiku Zařazení RIV Záznam nebyl označen do RIV Poddruh J Článek ve WOS Název Eukaryotic translation initiation factor 3 plays distinct roles at the mRNA entry and exit channels of the ribosomal preinitiation complex. Tvůrce(i) Aitken, C.E. (US)
Beznosková, Petra (MBU-M) RID
Vlčková, Vladislava (MBU-M) RID
Chiu, W.-L. (US)
Zhou, F. (US)
Valášek, Leoš Shivaya (MBU-M) RID, ORCID
Hinnebusch, A.G. (GB)
Lorsch, J. R. (US)Zdroj.dok. eLife. - : eLife - ISSN 2050-084X
Roč. 5, October 26 (2016), e20934Poč.str. 37 s. Jazyk dok. eng - angličtina Země vyd. GB - Velká Británie Klíč. slova S. cerevisiae ; biochemistry ; biophysics Vědní obor RIV EE - Mikrobiologie, virologie CEP GBP305/12/G034 GA ČR - Grantová agentura ČR Institucionální podpora MBU-M - RVO:61388971 UT WOS 000390805100001 EID SCOPUS 85006380078 DOI 10.7554/eLife.20934 Anotace Eukaryotic translation initiation factor 3 (eIF3) is a central player in recruitment of the pre-initiation complex (PIC) to mRNA. We probed the effects on mRNA recruitment of a library of S. cerevisiae eIF3 functional variants spanning its 5 essential subunits using an in vitro-reconstituted system. Mutations throughout eIF3 disrupt its interaction with the PIC and diminish its ability to accelerate recruitment to a native yeast mRNA. Alterations to the eIF3a CTD and eIF3b/i/g significantly slow mRNA recruitment, and mutations within eIF3b/i/g destabilize eIF2•GTP•Met-tRNAi binding to the PIC. Using model mRNAs lacking contacts with the 40S entry or exit channels, we uncovered a critical role for eIF3 requiring the eIF3a NTD, in stabilizing mRNA interactions at the exit channel, and an ancillary role at the entry channel requiring residues of the eIF3a CTD. These functions are redundant: defects at each channel can be rescued by filling the other channel with mRNA. Pracoviště Mikrobiologický ústav Kontakt Eliška Spurná, eliska.spurna@biomed.cas.cz, Tel.: 241 062 231 Rok sběru 2017
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