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Enantiomeric separation of triacylglycerols containing polyunsaturated fatty acids with 18 carbon atoms
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SYSNO ASEP 0464651 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Enantiomeric separation of triacylglycerols containing polyunsaturated fatty acids with 18 carbon atoms Tvůrce(i) Řezanka, Tomáš (MBU-M) ORCID
Nedbalová, L. (CZ)
Sigler, Karel (MBU-M) RIDZdroj.dok. Journal of Chromatography A. - : Elsevier - ISSN 0021-9673
Roč. 1467, October (2016), s. 261-269Poč.str. 9 s. Jazyk dok. eng - angličtina Země vyd. NL - Nizozemsko Klíč. slova Chiral triacylglycerols ; Enantiomers ; Regioisomers Vědní obor RIV EE - Mikrobiologie, virologie CEP GA14-00227S GA ČR - Grantová agentura ČR Institucionální podpora MBU-M - RVO:61388971 UT WOS 000385323800022 EID SCOPUS 84978805901 DOI 10.1016/j.chroma.2016.07.006 Anotace Regioisomers and enantiomers of triacylglycerols (TAGs) containing any combination of stearidonic (18:4n-3) and octadecapentaenoic (18:5n-3) acids were prepared by organic synthesis. Gradient polar organic liquid chromatography/high resolution atmospheric pressure chemical ionization-tandem mass spectrometry (NARP-LC/HRMS2-APCI) and chiral liquid chromatography were used for the separation and identification of molecular species of these TAGs. Further, NARP-LC and chiral LC were used to separate natural mixtures of TAGs obtained from the haptophyte alga Coccolithophora sp. cultivated in a salinity range from 7.5 to 60 parts per thousand. The ratio of regioisomers and enantiomers was found to change with increasing salinity of the culture medium. This can be explained by variable activity of acyltransferases in cells exposed to salt stress. (C) 2016 Elsevier B.V. All rights reserved. Pracoviště Mikrobiologický ústav Kontakt Eliška Spurná, eliska.spurna@biomed.cas.cz, Tel.: 241 062 231 Rok sběru 2017
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