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Cytotoxicity evaluation of large cyanobacterial strain set using selected human and murine in vitro cell models
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SYSNO ASEP 0464589 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Cytotoxicity evaluation of large cyanobacterial strain set using selected human and murine in vitro cell models Tvůrce(i) Hrouzek, Pavel (MBU-M) ORCID
Kapuscik, Alexandra (MBU-M)
Vacek, J. (CZ)
Voráčková, Kateřina (MBU-M)
Paichlová, Jindřiška (MBU-M)
Kosina, P. (CZ)
Voloshko, L. (RU)
Ventura, S. (IT)
Kopecký, Jiří (MBU-M) ORCIDZdroj.dok. Ecotoxicology and Enviromental Safety. - : Elsevier - ISSN 0147-6513
Roč. 124, February (2016), s. 177-185Poč.str. 9 s. Jazyk dok. eng - angličtina Země vyd. US - Spojené státy americké Klíč. slova Cytotoxicity ; Cyanobacteria ; Cell lines Vědní obor RIV EE - Mikrobiologie, virologie CEP GPP503/12/P614 GA ČR - Grantová agentura ČR GA14-18067S GA ČR - Grantová agentura ČR ED2.1.00/03.0110 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy LO1416 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy Institucionální podpora MBU-M - RVO:61388971 UT WOS 000369881000023 EID SCOPUS 84945367592 DOI 10.1016/j.ecoenv.2015.10.020 Anotace The production of cytotoxic molecules interfering with mammalian cells is extensively reported in cyanobacteria. These compounds may have a use in pharmacological applications; however, their potential toxicity needs to be considered. We performed cytotoxicity tests of crude cyanobacterial extracts in six cell models in order to address the frequency of cyanobacterial cytotoxicity to human cells and the level of specificity to a particular cell line. A set of more than 100 cyanobacterial crude extracts isolated from soil habitats (mainly genera Nostoc and Tolypothrix) was tested by MTT test for in vitro toxicity on the hepatic and non-hepatic human cell lines HepG2 and HeLa, and three cell systems of rodent origin: Yac-1, Sp-2 and Balb/c 3T3 fibroblasts. Furthermore, a subset of the extracts was assessed for cytotoxicity against primary cultures of human hepatocytes as a model for evaluating potential hepatotoxicity. Roughly one third of cyanobacterial extracts caused cytotoxic effects (i.e. viability < 75%) on human cell lines. Despite the sensitivity differences, high correlation coefficients among the inhibition values were obtained for particular cell systems. This suggests a prevailing general cytotoxic effect of extracts and their constituents. The non-transformed immortalized fibroblasts (Balb/c 3T3) and hepatic cancer line HepG2 exhibited good correlations with primary cultures of human hepatocytes. The presence of cytotoxic fractions in strongly cytotoxic extracts was confirmed by an activity-guided HPLC fractionation, and it was demonstrated that cyanobacterial cytotoxicity is caused by a mixture of components with similar hydrophobic/hydrophilic properties. The data presented here could be used in further research into in vitro testing based on human models for the toxicological monitoring of complex cyanobacterial samples. Pracoviště Mikrobiologický ústav Kontakt Eliška Spurná, eliska.spurna@biomed.cas.cz, Tel.: 241 062 231 Rok sběru 2017
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