Počet záznamů: 1
Acquisition of exogenous haem is essential for tick reproduction
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SYSNO ASEP 0463424 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Acquisition of exogenous haem is essential for tick reproduction Tvůrce(i) Perner, Jan (BC-A) RID, ORCID
Sobotka, Roman (MBU-M) RID, ORCID
Šíma, Radek (BC-A) RID, ORCID
Konvičková, Jitka (BC-A)
Sojka, Daniel (BC-A) RID, ORCID
de Oliveira, P.L. (BR)
Hajdušek, Ondřej (BC-A) RID, ORCID
Kopáček, Petr (BC-A) RID, ORCIDZdroj.dok. eLife. - : eLife - ISSN 2050-084X
Roč. 5, Mar 7 (2016), e12318Poč.str. 21 s. Forma vydání Online - E Jazyk dok. eng - angličtina Země vyd. GB - Velká Británie Klíč. slova biochemistry ; haem auxotrophy ; haem oxygenase ; haematophagy ; infectious disease ; iron metabolism ; microbiology ; ticks Vědní obor RIV EE - Mikrobiologie, virologie CEP GA13-11043S GA ČR - Grantová agentura ČR GP13-12816P GA ČR - Grantová agentura ČR GA14-33693S GA ČR - Grantová agentura ČR GP13-27630P GA ČR - Grantová agentura ČR LO1416 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy Institucionální podpora BC-A - RVO:60077344 ; MBU-M - RVO:61388971 UT WOS 000373891500001 EID SCOPUS 84964301646 DOI 10.7554/eLife.12318 Anotace Haem and iron homeostasis in most eukaryotic cells is based on a balanced flux between haem biosynthesis and haem oxygenase-mediated degradation. Unlike most eukaryotes, ticks possess an incomplete haem biosynthetic pathway and, together with other (non-haematophagous) mites, lack a gene encoding haem oxygenase. We demonstrated, by membrane feeding, that ticks do not acquire bioavailable iron from haemoglobin-derived haem. However, ticks require dietary haemoglobin as an exogenous source of haem since, feeding with haemoglobin-depleted serum led to aborted embryogenesis. Supplementation of serum with haemoglobin fully restored egg fertility. Surprisingly, haemoglobin could be completely substituted by serum proteins for the provision of amino-acids in vitellogenesis. Acquired haem is distributed by haemolymph carrier protein(s) and sequestered by vitellins in the developing oocytes. This work extends, substantially, current knowledge of haem auxotrophy in ticks and underscores the importance of haem and iron metabolism as rational targets for anti-tick interventions. Pracoviště Biologické centrum (od r. 2006) Kontakt Dana Hypšová, eje@eje.cz, Tel.: 387 775 214 Rok sběru 2017
Počet záznamů: 1