Počet záznamů: 1
Distribution of CD46 and β1 integrin molecules with respect to different membrane structures of the sperm head
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SYSNO ASEP 0463213 Druh ASEP C - Konferenční příspěvek (mezinárodní konf.) Zařazení RIV Záznam nebyl označen do RIV Název Distribution of CD46 and β1 integrin molecules with respect to different membrane structures of the sperm head Tvůrce(i) Šebková, Nataša (BTO-N)
Frolíková, Michaela (BTO-N) ORCID
Děd, Lukáš (BTO-N) RID
Dvořáková-Hortová, Kateřina (BTO-N)Celkový počet autorů 4 Zdroj.dok. Programme and Abstract Book. - Prague : Czech Society for Cell Biology, z.s, 2016 Rozsah stran s. 139-139 Poč.str. 1 s. Akce 12th international Congress of Cell Biology Datum konání 21.07.2016-25.07.2016 Místo konání Praha Země CZ - Česká republika Typ akce WRD Jazyk dok. eng - angličtina Země vyd. CZ - Česká republika Klíč. slova CD46 ; β1 integrin ; acrosome reaction ; latrunculin A ; sperm-egg fussion ; Proximity ligation assay CEP GA14-05547S GA ČR - Grantová agentura ČR ED1.1.00/02.0109 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy Institucionální podpora BTO-N - RVO:86652036 Anotace CD46 protein plays an important role during fertilization and its role is associated with acrosome stability. CD46 is probably involved in signalling pathways triggering the acrosome reaction (AR). It also associates through membrane integrins with specific MAP kinases involved in the AR. Our aim was to monitor the dynamics of relocation of CD46 and β1 integrin during sperm maturation and its preparation for the fertilization. The dependence of this localization changes on the dynamic of actin cytoskeleton was studied. Our results show the changes in the localization of these proteins associated with the AR and their co-localization was observed using proximity ligation assay. After the AR CD46 and β1 integrin spreads across the sperm head, entering the post-acrosomal compartment, and permeates the borders of different domains. It was shown previously that actin dynamics is necessary for acrosome reaction-associated translocation of Izumo1 protein that is required for sperm-egg fusion. Therefore Latrunculin A was used during sperm incubation. The co-incubation of capacitated sperm with Latrunculin A leads to a decrease of the percentages of sperm, which express relocation pattern after induced AR. 3D models and visualizations of potential membrane processes responsible for the relocation of proteins from the acrosomal area to the other compartments of the sperm head were prepared. Our results deliver new information that proteins CD46 and β1 integrin undergo dynamic relocation towards the sites of sperm-egg fusion during the AR in vitro. The inhibitor of actin dynamics abrogates significantly the AR-associated changes in proteins localization. We speculate that this relocation is of importance for the successful sperm-egg interaction, adhesion and subsequent gamete fusion. Pracoviště Biotechnologický ústav Kontakt Monika Kopřivová, Monika.Koprivova@ibt.cas.cz, Tel.: 325 873 700 Rok sběru 2017
Počet záznamů: 1