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Use of In Vitro Transcription System for Analysis of Corynebacterium glutamicum Promoters Recognized by Two Sigma Factors
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SYSNO ASEP 0462752 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Use of In Vitro Transcription System for Analysis of Corynebacterium glutamicum Promoters Recognized by Two Sigma Factors Tvůrce(i) Šilar, Radoslav (MBU-M)
Holátko, Jiří (MBU-M)
Rucká, Lenka (MBU-M)
Rapoport, Andrey (MBU-M)
Dostálová, Hana (MBU-M)
Kadeřábková, Pavla (MBU-M)
Nešvera, Jan (MBU-M) RID
Pátek, Miroslav (MBU-M) RID, ORCIDZdroj.dok. Current Microbiology. - : Springer - ISSN 0343-8651
Roč. 73, č. 3 (2016), s. 401-408Poč.str. 8 s. Jazyk dok. eng - angličtina Země vyd. US - Spojené státy americké Klíč. slova GENE-EXPRESSION ; REGULATORY NETWORK ; BACILLUS-SUBTILIS Vědní obor RIV EE - Mikrobiologie, virologie Institucionální podpora MBU-M - RVO:61388971 UT WOS 000381114200015 EID SCOPUS 84982792270 DOI 10.1007/s00284-016-1077-x Anotace Promoter activities in Corynebacterium glutamicum strains with deletions of genes encoding sigma factors of RNA polymerase suggested that transcription from some promoters is controlled by two sigma factors. To prove that different sigma factors are involved in the recognition of selected Corynebacterium glutamicum promoters, in vitro transcription system was applied. It was found that a typical housekeeping promoter Pper interacts with the alternative sigma factor sigma(B) in addition to the primary sigma factor sigma(A). On the other way round, the sigma(B)-dependent promoter of the pqo gene that is expressed mainly in the stationary growth phase was active also with sigma(A). Some promoters of genes involved in stress responses (P1clgR, P2dnaK, and P2dnaJ2) were found to be recognized by two stress-responding sigma factors, sigma(H) and sigma(E). Pracoviště Mikrobiologický ústav Kontakt Eliška Spurná, eliska.spurna@biomed.cas.cz, Tel.: 241 062 231 Rok sběru 2017
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