Počet záznamů: 1

Photo-induced DNA cleavage and cytotoxicity of a ruthenium(II) arene anticancer complex

SYSNO ASEP	0461985
Druh ASEP	J - Článek v odborném periodiku
Zařazení RIV	J - Článek v odborném periodiku
Poddruh J	Článek ve WOS
Název	Photo-induced DNA cleavage and cytotoxicity of a ruthenium(II) arene anticancer complex
Tvůrce(i)	Brabec, Viktor (BFU-R)_{RID, ORCID} Prachařová, J. (CZ) Štěpánková, Jana (BFU-R) Sadler, P. J. (GB) Kašpárková, Jana (BFU-R)_{RID, ORCID}
Celkový počet autorů	5
Zdroj.dok.	Journal of Inorganic Biochemistry. - : Elsevier - ISSN 0162-0134 Roč. 160, JUL2016 (2016), s. 149-155
Poč.str.	7 s.
Forma vydání	Tištěná - P
Jazyk dok.	eng - angličtina
Země vyd.	US - Spojené státy americké
Klíč. slova	Ruthenium anticancer complex ; DNA cleavage ; Phototoxicity
Vědní obor RIV	BO - Biofyzika
CEP	GA14-21053S GA ČR - Grantová agentura ČR
	LD14019 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy
Institucionální podpora	BFU-R - RVO:68081707
UT WOS	000378965200017
DOI	10.1016/j.jinorgbio.2015.12.029
Anotace	We report DNA cleavage by ruthenium(II) arene anticancer complex [(eta(6)-p-terp)Ru-II(en)Cl](+) (p-terp = paraterphenyl, en = 1,2-diaminoethane, complex 1) after its photoactivation by UVA and visible light, and the toxic effects of photoactivated 1 in cancer cells. It was shown in our previous work (T. Bugarcic et al., J. Med. Chem. 51 (2008) 5310-5319) that this complex exhibits promising toxic effects in several human tumor cell lines and concomitantly its DNA binding mode involves combined intercalative and monofunctional (coordination) binding modes. We demonstrate in the present work that when photoactivated by UVA or visible light, 1 efficiently photocleaves DNA, also in hypoxic media. Studies of the mechanism underlying DNA cleavage by photoactivated 1 reveal that the photocleavage reaction does not involve generation of reactive oxygen species (ROS), although contribution of singlet oxygen (O-1(2)) to the DNA photocleavage process cannot be entirely excluded. Notably, the mechanism of DNA photocleavage by 1 appears to involve a direct modification of mainly those guanine residues to which 1 is coordinatively bound.
Pracoviště	Biofyzikální ústav
Kontakt	Jana Poláková, polakova@ibp.cz, Tel.: 541 517 244
Rok sběru	2017

Počet záznamů: 1