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Quantitative proteomics of the tobacco pollen tube secretome identifies novel pollen tube guidance proteins important for fertilization
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SYSNO ASEP 0461616 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Quantitative proteomics of the tobacco pollen tube secretome identifies novel pollen tube guidance proteins important for fertilization Tvůrce(i) Hafidh, Said (UEB-Q) RID, ORCID
Potěšil, D. (CZ)
Fíla, Jan (UEB-Q) RID, ORCID
Čapková, Věra (UEB-Q) RID
Zdráhal, Z. (CZ)
Honys, David (UEB-Q) RID, ORCIDČíslo článku 81 Zdroj.dok. Genome Biology - ISSN 1465-6906
Roč. 17, MAY 3 (2016)Poč.str. 29 s. Jazyk dok. eng - angličtina Země vyd. US - Spojené státy americké Klíč. slova Protein secretion ; Pollen tube guidance ; Cell-cell signaling Vědní obor RIV EB - Genetika a molekulární biologie CEP GA15-22720S GA ČR - Grantová agentura ČR GA14-32292S GA ČR - Grantová agentura ČR GA15-16050S GA ČR - Grantová agentura ČR LD14109 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy LM2015043 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy ED1.1.00/02.0068 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy LQ1601 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy Institucionální podpora UEB-Q - RVO:61389030 UT WOS 000375962000001 DOI 10.1186/s13059-016-0928-x Anotace We performed genome-wide quantitative liquid chromatography-tandem mass spectrometry analysis of a pistil-stimulated pollen tube secretome and identified 801 pollen tube-secreted proteins. Interestingly, in silico analysis reveals that the pollen tube secretome is dominated by proteins that are secreted unconventionally, representing 57 % of the total secretome. In support, we show that an unconventionally secreted protein, translationally controlled tumor protein, is secreted to the apoplast. Remarkably, we discovered that this protein could be secreted by infiltrating through the initial phases of the conventional secretory pathway and could reach the apoplast via exosomes, as demonstrated by co-localization with Oleisin1 exosome marker. We demonstrate that translationally controlled tumor protein-knockdown Arabidopsis thaliana plants produce pollen tubes that navigate poorly to the target ovule and that the mutant allele is poorly transmitted through the male. Further, we show that regulators of the endoplasmic reticulum-trans-Golgi network protein secretory pathway control secretion of Nicotiana tabacum Pollen tube-secreted cysteine-rich protein 2 and Lorelei-like GPI-anchor protein 3 and that a regulator of endoplasmic reticulum-trans-Golgi protein translocation is essential for pollen tube growth, pollen tube guidance and ovule-targeting competence. Pracoviště Ústav experimentální botaniky Kontakt David Klier, knihovna@ueb.cas.cz, Tel.: 220 390 469 Rok sběru 2017
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