Počet záznamů: 1
Repetitive Inductions of Bioluminescence of Pseudomonas putida TVA8 Immobilized by Adsorption on Optical Fibre.
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SYSNO ASEP 0461501 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Repetitive Inductions of Bioluminescence of Pseudomonas putida TVA8 Immobilized by Adsorption on Optical Fibre. Tvůrce(i) Zajíc, J. (CZ)
Bittner, M. (CZ)
Brányik, T. (CZ)
Solovyev, Andrey (UCHP-M) RID, ORCID, SAI
Šabata, Stanislav (UCHP-M) RID, ORCID, SAI
Kuncová, Gabriela (UCHP-M) RID, SAI
Pospíšilová, M. (CZ)Zdroj.dok. Chemical Papers. - : Springer - ISSN 0366-6352
Roč. 70, č. 7 (2016), s. 877-887Poč.str. 11 s. Jazyk dok. eng - angličtina Země vyd. SK - Slovensko Klíč. slova whole-cell biosensor ; bioluminiscent bioreporter ; optical fibre sensor Vědní obor RIV CC - Organická chemie Institucionální podpora UCHP-M - RVO:67985858 UT WOS 000379757200002 EID SCOPUS 85010288574 DOI 10.1515/chempap-2016-0031 Anotace Physico-chemical models of the interactions of cells with solid surfaces, which use contact angles and zeta potentials, indicated more facile adsorption of cells of Pseudomonas putida TVA8 on the quartz surface after its treatment with 3-aminopropyltriethoxysilane (APTES). A whole-cell optical fibre sensor of toluene was prepared by the adsorption of P. putida TVA8, bacteria producing light in contact with toluene on the wider end of APTES-treated quartz tapered optical fibre. The results of the measurements of luminescence from both sides of the layer of adsorbed cells were compared. Over the 135 days trial, the fibre biosensor was repetitively induced with toluene solution (26.5 mg L-1) 68 times. The intensities of bioluminescence gradually decreased due to release of the adsorbed cells and they were only temporarily restored by the addition of nutrients. The intensities of bioluminescence induced with contaminated ground water were lower than in the mineral medium (MSM) with the same content of toluene. Pracoviště Ústav chemických procesů Kontakt Eva Jirsová, jirsova@icpf.cas.cz, Tel.: 220 390 227 Rok sběru 2017
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