Počet záznamů: 1
Sensing microorganisms in the gut triggers the immune response in Eisenia andrei earthworms
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SYSNO ASEP 0460105 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Sensing microorganisms in the gut triggers the immune response in Eisenia andrei earthworms Tvůrce(i) Dvořák, Jiří (MBU-M) RID, ORCID
Roubalová, Radka (MBU-M) RID, ORCID
Procházková, Petra (MBU-M) RID, ORCID
Rossmann, Pavel (MBU-M)
Škanta, František (MBU-M) RID
Bilej, Martin (MBU-M) RID, ORCIDZdroj.dok. Developmental and Comparative Immunology. - : Elsevier - ISSN 0145-305X
Roč. 57, APR 2016 (2016), s. 67-74Poč.str. 8 s. Jazyk dok. eng - angličtina Země vyd. GB - Velká Británie Klíč. slova Earthworm ; Mucosal immunity ; CCF Vědní obor RIV EE - Mikrobiologie, virologie CEP ED1.1.00/02.0109 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy Institucionální podpora MBU-M - RVO:61388971 UT WOS 000370091300008 EID SCOPUS 84952802000 DOI 10.1016/j.dci.2015.12.001 Anotace The tube-within-tube body plan of earthworms is appropriate for studying the interactions of microorganisms with the immune system of body cavities such as the digestive tract and coelom. This study aims to describe the immune response on the molecular and cellular level in the coelomic cavity and the gut of the earthworm Eisenia andrei after experimental microbial challenge by administering two bacterial strains (Escherichia coli and Bacillus subtilis) or yeast Saccharomyces cerevisiae to the environment. The changes in mRNA levels of defense molecules (pattern recognition receptor CCF, lysozyme, fetidin/lysenins) in the coelomocytes and gut tissue were determined by quantitative PCR. The immune response at a cellular level was captured in histological sections, and the expression of CCF was localized using in situ hybridization. Coelomocytes respond to the presence of bacteria in the coelomic cavity by increasing the mRNA levels of defense molecules, especially CCF. The immune response in gut tissue is less affected by microbial stimulation because the epithelial cells of gut exhibit basically strong mRNA synthesis of ccf as a defense against the continuous microbial load in the gut lumen. Pracoviště Mikrobiologický ústav Kontakt Eliška Spurná, eliska.spurna@biomed.cas.cz, Tel.: 241 062 231 Rok sběru 2017
Počet záznamů: 1