Počet záznamů: 1

LBP/BPI homologue in Eisenia andrei earthworms

SYSNO ASEP	0460102
Druh ASEP	J - Článek v odborném periodiku
Zařazení RIV	J - Článek v odborném periodiku
Poddruh J	Článek ve WOS
Název	LBP/BPI homologue in Eisenia andrei earthworms
Tvůrce(i)	Škanta, František (MBU-M)_RID Procházková, Petra (MBU-M)_{RID, ORCID} Roubalová, Radka (MBU-M)_{RID, ORCID} Dvořák, Jiří (MBU-M)_{RID, ORCID} Bilej, Martin (MBU-M)_{RID, ORCID}
Zdroj.dok.	Developmental and Comparative Immunology. - : Elsevier - ISSN 0145-305X Roč. 54, č. 1 (2016), s. 1-6
Poč.str.	6 s.
Jazyk dok.	eng - angličtina
Země vyd.	GB - Velká Británie
Klíč. slova	Earthworm ; Eisenia ; LBP/BPI
Vědní obor RIV	EC - Imunologie
CEP	ED1.1.00/02.0109 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy
	EE2.3.30.0003 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy
Institucionální podpora	MBU-M - RVO:61388971
UT WOS	000364883100001
EID SCOPUS	84940417074
DOI	10.1016/j.dci.2015.08.008
Anotace	LBP/BPIs are pattern recognition receptors that are often present in vertebrates and in invertebrates, and they play a defense role against pathogens. We have identified 1698 bp cDNA sequence from the Eisenia andrei earthworm with predicted amino acid sequence that shares homology with the LBP/BPI family (EaLBP/BPI). Sequence analysis of EaLBP/BPI proved the existence of two conserved domains with the potential ability to bind LPS. The predicted molecular mass of the EaLBP/BPI protein is 53.5 kDa, and its high basicity (pI 9.8) is caused by its high arginine content. Constitutive transcription of the Ealbp/bpi gene was shown in all tested tissues, with the highest level in coelomocytes and seminal vesicles; the lowest level was detected in the intestine. On the contrary, another earthworm LPS-binding molecule CCF (coelomic cytolytic factor) was expressed only in the intestine and coelomocytes. In E. andrei coelomocytes, the transcription of Ealbp/bpi gene was up-regulated in response to bacterial stimulation, reaching a maximum at 8 and 16 h post stimulation with Bacillus subtilis and Escherichia coli, respectively.
Pracoviště	Mikrobiologický ústav
Kontakt	Eliška Spurná, eliska.spurna@biomed.cas.cz, Tel.: 241 062 231
Rok sběru	2017

Počet záznamů: 1