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Composite particles formed by complexation of poly(methacrylic acid) - stabilized magnetic fluid with chitosan: Magnetic material for bioapplications
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SYSNO ASEP 0460075 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Composite particles formed by complexation of poly(methacrylic acid) - stabilized magnetic fluid with chitosan: Magnetic material for bioapplications Tvůrce(i) Šafařík, Ivo (BC-A) RID, ORCID
Štěpánek, M. (CZ)
Uchman, M. (CZ)
Šlouf, Miroslav (UMCH-V) RID, ORCID
Baldíková, E. (CZ)
Nýdlová, L. (CZ)
Pospíšková, K. (CZ)
Šafaříková, Miroslava (BC-A) RIDZdroj.dok. Materials Science & Engineering C-Materials for Biological Applications. - : Elsevier - ISSN 0928-4931
Roč. 67, October (2016), s. 486-492Poč.str. 7 s. Jazyk dok. eng - angličtina Země vyd. NL - Nizozemsko Klíč. slova chitosan ; immobilization ; lipase ; magnetic fluid ; poly(methacrylic acid) Vědní obor RIV CD - Makromolekulární chemie Vědní obor RIV – spolupráce Ústav makromolekulární chemie - Makromolekulární chemie CEP TE01020118 GA TA ČR - Technologická agentura ČR LO1507 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy Institucionální podpora BC-A - RVO:60077344 ; UMCH-V - RVO:61389013 UT WOS 000378952800055 EID SCOPUS 84971247668 DOI 10.1016/j.msec.2016.05.017 Anotace A simple procedure for the synthesis of magnetic fluid (ferrofluid) stabilized by poly(methacrylic acid) has been developed. This ferrofluid was used to prepare a novel type of magnetically responsive chitosan-based composite material. Both ferrofluid and magnetic chitosan composite were characterized by a combination of microscopy (optical microscopy, TEM, SEM), scattering (static and dynamic light scattering, SANS) and spectroscopy (FTIR) techniques. Magnetic chitosan was found to be a perspective material for various bioapplications, especially as a magnetic carrier for immobilization of enzymes and cells. Lipase from Candida rugosa was covalently attached after cross-linking and activation of chitosan using glutaraldehyde. Baker's yeast cells (Saccharomyces cerevisiae./.) were incorporated into the chitosan composite during its preparation; both biocatalysts were active after reaction with appropriate substrates. Pracoviště Biologické centrum (od r. 2006) Kontakt Dana Hypšová, eje@eje.cz, Tel.: 387 775 214 Rok sběru 2017
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